Protein Info for HP15_463 in Marinobacter adhaerens HP15

Annotation: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 768 PF13185: GAF_2" amino acids 15 to 155 (141 residues), 61.6 bits, see alignment E=2.8e-20 PF13492: GAF_3" amino acids 17 to 156 (140 residues), 45.3 bits, see alignment E=3.5e-15 PF01590: GAF" amino acids 17 to 154 (138 residues), 71.1 bits, see alignment E=4.2e-23 PF05524: PEP-utilisers_N" amino acids 180 to 302 (123 residues), 95.5 bits, see alignment E=7.9e-31 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 181 to 738 (558 residues), 447.7 bits, see alignment E=2.8e-138 PF00391: PEP-utilizers" amino acids 328 to 400 (73 residues), 64.1 bits, see alignment E=2.1e-21 PF02896: PEP-utilizers_C" amino acids 429 to 714 (286 residues), 343.2 bits, see alignment E=3.6e-106

Best Hits

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 94% identity to maq:Maqu_0815)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PMU9 at UniProt or InterPro

Protein Sequence (768 amino acids)

>HP15_463 fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain protein (Marinobacter adhaerens HP15)
MLSILRSLVQEVNSARDLQEALDIIVSRVQKAMGTEVCSVYLLDPATNRYILMATEGLYR
KAVGQVSLAYSEGLVGLVGSREEPINLEDAPSHPRYRYFPETGEERFRSFLGVPIIHHRR
VLGVLVVQQRESSRCFDEGEEAFLVTVSAQLAGVIAHSEATGAISGLSLTGEEAHDVSFN
GVPGAPGVAIGNGVVVYPSADLDVVPEKPTDDIDQELALFGASVKAVREDIERVAKRLAS
QLRPEEQALFDVYLRMLGDDAMPGEVSNKIREGIWAQGALKQVVQQYIRHFEMMDDHYLQ
ERAVDIRDLGRRLLSHLQEGEQKHLNYPERTVLVSEELTPAMLGEVPKGQLVGLVSVKGS
SNSHVAILARAMGVPTVMGLVDIPVNQLDGKELIVDGFEGQIFASPSADLRSYYQAICDE
EDELIRGLEVLKDKPCETTDHHRVSLLVNTGLMTDVVRSLSHGAEGIGLYRTEVPFMIKD
RFPSEQEQREYYREQLEAFAPSPVTMRTLDIGGDKALTYFPIQEENPFLGWRGIRVTLDH
PEIFLVQVRAMLKASEGLNNLQIMLPMISNISEVEESLHLIYRVYHEVREEGYDIHMPKV
GVMVEIPAAVYQIRELADRVDFLSVGSNDLTQYLLAVDRNNPRVAQLYHSYHPAVLQALV
RIAQDAHSVGKPVGICGELAGDPGGALLLMAMGYDSLSMNAASLPKVKSVIRSVSREWAI
QLLEDVLLLDSPHVIKSCVDLALRNAGFGRYLRPGKSTAMAVSEAAVS