Protein Info for HP15_457 in Marinobacter adhaerens HP15
Annotation: 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 75% identical to METE_MARHV: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)
KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 75% identity to maq:Maqu_0809)MetaCyc: 56% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]
Predicted SEED Role
"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- L-methionine biosynthesis III (4/4 steps found)
- S-adenosyl-L-methionine salvage II (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- seleno-amino acid biosynthesis (plants) (3/5 steps found)
- S-adenosyl-L-methionine salvage I (2/4 steps found)
- superpathway of seleno-compound metabolism (6/19 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.1.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PMU3 at UniProt or InterPro
Protein Sequence (759 amino acids)
>HP15_457 5-methyltetrahydropteroyltriglutamate- homocysteine S-methyltransferase (Marinobacter adhaerens HP15) MVTTHNLGFPRIGARRELKFAQEAFWKGRISEEDLQISGADLRRRHWQNQRPLDRVPVGD FSFYDQILDMSVTLGNLPERVNDTKGSELDRYFRVARGRSGQESACCGVQAGEMTKWFDT NYHYIVPEFHQNTRFRLNGNRLIDQIDEARAQGVTPKPVIIGPVTYLWLGKAKDDSDRLL LLESLLPVYSELLELLADRGVEWVQVDEPALVTDLDADWRHAFSLAYHHLKTSTPKLLVT TYFGELRENLQLACELPVAGLHLDAISAPQEVPRVVDWLPPHKQLSLGVINGRNIWRSDL EKTLDWLEPVYEKLGNRLWLAPSCSLIHVPVDLTSEDKLDPEIRSWLAFAVQKLDELKTL ATALNNGRAAVRKQLAESTVAVNSRAKSERVHSASVQARLADVSPALGQRQSRFPDRIAI QQRKLGLPRFPTTTIGSFPQTRDIRQTRLQFRKGELTEPQYNARIREEVRRCIVEQEALG LDVLVHGEAERNDMVEYFGEQLDGYTFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEW IRYAQSLTKKPLKGMLTGPVTILNWSFVRDDQPRRETCLQLALAIREEVLDLEASGARII QIDEAALREGLPLRQADWKNYLDWAIESFRISANGVRDETQIHTHMCYSEFNDIIEAIAR MDADVITIETSRSDMELLDAFRGFHYPNDIGPGVYDIHSPNIPDSEQIRSLMIKAAERIP AERLWVNPDCGLKTRQWEEVRPALKSMVAAARELRETIQ