Protein Info for Psest_0467 in Pseudomonas stutzeri RCH2

Annotation: lipid A export permease/ATP-binding protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 83 to 106 (24 residues), see Phobius details amino acids 162 to 180 (19 residues), see Phobius details amino acids 185 to 202 (18 residues), see Phobius details amino acids 267 to 290 (24 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 13 to 597 (585 residues), 810.8 bits, see alignment E=3.2e-248 PF00664: ABC_membrane" amino acids 38 to 309 (272 residues), 166.8 bits, see alignment E=8.5e-53 PF00005: ABC_tran" amino acids 374 to 524 (151 residues), 123.1 bits, see alignment E=1.4e-39

Best Hits

Swiss-Prot: 79% identical to MSBA_PSEF5: Lipid A export ATP-binding/permease protein MsbA (msbA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 88% identity to psa:PST_3830)

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA" in subsystem ZZ gjo need homes

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIE4 at UniProt or InterPro

Protein Sequence (605 amino acids)

>Psest_0467 lipid A export permease/ATP-binding protein MsbA (Pseudomonas stutzeri RCH2)
MANSTQQSSMKVYLRLLRYVVPYWGLFTISLVGFLIFASTQPMLGYMLKFFVDGLNNPES
SFFGQVPYLQEHSWLAELKLLQAVPLLIVFIALMQGIGSFLGNFFLAKVSLGLVHDLRLA
LFNNMLTLPNRYFDSHNSGHLISRITYNVTMVTGAATDAIKVVVREGMTVIFLFATLLWM
NWKLTLVMLAILPVIGFMVSSASKKFRKQSKKIQLAMGDVTHVASETIQGYRVVRSFGGE
PYERNRFLTASSENTAKQLRMVKTNAVYTPSLQLVIYSAMAVLMFLVLLLRGDASAGDLV
AYITLAGLLPKPIRQLSEVSSTIQKGVAGAESIFEQLDEEPEIDQGTLEREAVSGHLEVR
NLDFVYPGTDKQVLRDISFTIEPGQMVALVGRSGSGKSTLANLIPRFYHHEAGQILLDGA
DVESYTLRNLRRHIALVTQQVTLFNDTVANNIAYGDLAGAPLDDIRRAARDAYADEFIEQ
MPEGYQTLVGENGVLLSGGQRQRLAIARALLKNSPVLILDEATSALDTESERHIQGALDH
VMQGRTTLVIAHRLSTIEKADQILVMEQGRIVERGTHAELLAVNGAYARLHATQFKDEVP
EEQES