Protein Info for PGA1_c04710 in Phaeobacter inhibens DSM 17395

Annotation: (dimethylallyl)adenosine tRNA methylthiotransferase MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 6 to 438 (433 residues), 496.3 bits, see alignment E=8.7e-153 PF00919: UPF0004" amino acids 6 to 105 (100 residues), 113.8 bits, see alignment E=4.7e-37 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 6 to 436 (431 residues), 486.1 bits, see alignment E=9.2e-150 PF04055: Radical_SAM" amino acids 155 to 326 (172 residues), 93.8 bits, see alignment E=2.1e-30 PF01938: TRAM" amino acids 379 to 438 (60 residues), 22.5 bits, see alignment E=1.3e-08

Best Hits

Swiss-Prot: 93% identical to MIAB_RUEST: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 93% identity to sit:TM1040_0185)

MetaCyc: 51% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EWK5 at UniProt or InterPro

Protein Sequence (440 amino acids)

>PGA1_c04710 (dimethylallyl)adenosine tRNA methylthiotransferase MiaB (Phaeobacter inhibens DSM 17395)
MSAPKKLFIKTYGCQMNVYDSERMAEALGGEGYVETKSPDDADMILLNTCHIREKAAEKV
YSELGRFKGLKAEKPDLKIGVAGCVAQAEGQEIMRRQPLVDLVVGPQSYHRLPEMEAKAR
EGEKVLDTDFPEEDKFEKLKNRPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPA
DRILREAQDLVERGVREITLLGQNVNAYHGAGPNGDMTLAQLIWELDKVDGLERIRFTTS
HPNDMQDDLIEAHGTCAKLMPYLHLPVQAGSDKILKRMNRAHTAESYLRLIERIRAARPD
ILISGDFIVGFPEETEADFQATLDLVEEVKYGTAYSFKYSTRPGTPAAERPQVDPKEADD
RLQRLQALLTTQQREVQDSMVGRDVTVLFEKAGRFPGQMVGKSDYLHAVHVADCDRAIGD
LARVRILSSGANSLAGALID