Protein Info for PGA1_c04630 in Phaeobacter inhibens DSM 17395
Annotation: 2-nitropropane dioxygenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to NMO_PARPJ: Nitronate monooxygenase (Bphyt_4144) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)
KEGG orthology group: K00459, nitronate monooxygenase [EC: 1.13.12.16] (inferred from 63% identity to bmu:Bmul_1549)Predicted SEED Role
"Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)" in subsystem Fatty Acid Biosynthesis FASII (EC 1.3.1.9)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (49/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- superpathway of unsaturated fatty acids biosynthesis (E. coli) (18/20 steps found)
- superpathway of fatty acid biosynthesis I (E. coli) (15/16 steps found)
- oleate biosynthesis IV (anaerobic) (13/14 steps found)
- biotin biosynthesis I (13/15 steps found)
- (5Z)-dodecenoate biosynthesis I (6/6 steps found)
- palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
- gondoate biosynthesis (anaerobic) (4/4 steps found)
- 8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
- (5Z)-dodecenoate biosynthesis II (5/6 steps found)
- stearate biosynthesis II (bacteria and plants) (5/6 steps found)
- 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
- cis-vaccenate biosynthesis (4/5 steps found)
- fatty acid elongation -- saturated (4/5 steps found)
- anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
- even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
- odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
- alkylnitronates degradation (1/2 steps found)
- streptorubin B biosynthesis (20/34 steps found)
- mycolate biosynthesis (21/205 steps found)
- superpathway of mycolate biosynthesis (22/239 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.1.9
Use Curated BLAST to search for 1.13.12.16 or 1.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7ETZ9 at UniProt or InterPro
Protein Sequence (364 amino acids)
>PGA1_c04630 2-nitropropane dioxygenase (Phaeobacter inhibens DSM 17395) MSSISTSAALMDRLGISLPILQSPMAGVSTPQMAAAVSNAGGLGALGIGAASVEQAEKML LQTAALTGRPFNVNVFCHKPATADPVREAGWLQHLSPAFQEFGGKPPQGLREIYTSFCVD RDMQDMLIAQRPAVVSFHLGLPSQDVIAALKAAGVVLLATATSLAEARSLERAGIDAIVA QGWEAGGHRGIFDPAGEDGRLQTLPLVRELVGALALPVIAAGGMMTGADIAEALAAGAAA AQLGTAFIACPESSADAGYRAALLADDPETEMTAVISGRPARCLSNRFTDWGRQNRDAGI PAYPIAYDAGKALNAAAKNRDEFGYGAQWAGQGAAHARVMPTAELVAMLAQELSVAQRED RAPE