Protein Info for HP15_435 in Marinobacter adhaerens HP15

Annotation: glutamate-ammonia-ligase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 968 PF03710: GlnE" amino acids 44 to 290 (247 residues), 274.2 bits, see alignment E=1e-85 amino acids 571 to 823 (253 residues), 293.6 bits, see alignment E=1.2e-91 PF08335: GlnD_UR_UTase" amino acids 314 to 452 (139 residues), 107.8 bits, see alignment E=4.7e-35 amino acids 850 to 935 (86 residues), 26.4 bits, see alignment E=6.3e-10

Best Hits

Swiss-Prot: 52% identical to GLNE_NITOC: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / NCIMB 11848 / C-107)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 81% identity to maq:Maqu_0776)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PMS1 at UniProt or InterPro

Protein Sequence (968 amino acids)

>HP15_435 glutamate-ammonia-ligase adenylyltransferase (Marinobacter adhaerens HP15)
MSEPWHSLPKPLADDVAGCWESVFPEGVPAWLTSPEGMDDTVVATAISRSLFLRQTLERH
ADQVEALMASRSLTEPTTPDYLESRWQEYLSEVDGEPGLHSALRQFRMETQFRIIWRDLM
RWADLAETMAATSVFADICIDGALSWLYQDACEQYGTPRGADPVTGQETDQKMVVLGMGK
LGGRELNVSSDIDLIFAFPSKGETVGGRRSLDNQQFFVRLGQRLIQALDQITADGFVFRV
DMRLRPYGQSGALALSFAALETYYQDQGRDWERYAMVKARVVAGDQRAGQVLMETLRPFV
YRKYIDFSAFESLRSMKAMIGREVRRKGLENNIKLGSGGIREIEFVVQAFQLIRGGRDRE
LQQRELLVILKELEELELLPSPVVGELRDAYVFLRNLEHALQGMEDKQTQLLPEDELSRT
RVATIMDFENWQACEQCLREHRERVATHFSNIITTEDDEDEGQGALEEGWFELWLSEMEE
AAAADWLKSRGYEDPGATLAELAELRENRTVQNLQTQGRRRLNQFMPMLLEALTQVDNPS
ETLSRVLQLVEAILRRTAYMVLLLENPGACTQLVRLCSESPWIARQLAETPLLLDELLNA
ESLYSPPAKAELQDDLRQQMLRIPFEDLEEQMESLRHFKKAHILRVAASELKGTLPLMKV
SDYLTWIAEVVLDHVVDVAFANLVSRHGYPRREDGSACETDFAIIGYGKLGGIELGYTSD
LDLVFVHKADPELATDGEKPIDNAVFYTRLGQRIVHILNTQTPSGQLYEVDMRLRPSGNS
GLLVSTLQAFEKYQREDAWTWEHQALARARGVAGCTETLEAFESIRHEILCQPRDREKLR
NEVVEMREKMRASLATPESRQDEVFHIKHDTGGIVDIEFIVQYLMLAWSAEHPELTQWSD
NIRQMEELGRAGVMAVEDAEKLRETFIALRSTIHRRALQNLNSQVEGDAFSQERDYIRAM
WKKVMIEA