Protein Info for PGA1_c04560 in Phaeobacter inhibens DSM 17395

Annotation: putative fumarate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00206: Lyase_1
  • amino acids 24 to 298 (275 residues), 114.2 bits, E=4.3e-37

Best Hits

KEGG orthology group: K01857, 3-carboxy-cis,cis-muconate cycloisomerase [EC: 5.5.1.2] (inferred from 61% identity to sil:SPOA0432)

Predicted SEED Role

"3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 5.5.1.2)

MetaCyc Pathways

• protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
• aromatic compounds degradation via β-ketoadipate (7/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
• superpathway of aerobic toluene degradation (11/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.5.1.2

Sequence Analysis Tools

See I7EWJ4 at UniProt or InterPro

Protein Sequence (449 amino acids)

>PGA1_c04560 putative fumarate lyase (Phaeobacter inhibens DSM 17395)
MAASLFDSQLYASLFSAGDVSRLFSDSAEIRAMLLVEGALAKAQGKLGLIPEDSAAAIGR
AVMEVALDPGALTQAAGQNGVPVPGLVSALRDEMNAPEHAQYVHWGATSQDIMDSALMLR
LRQALAAVETDLLILLTQLSDMADTHANLPMAARTYGQLATPTSFGAVVAAWGQPLAALL
EELPTLRQTCLLVSLSGAAGTSSALGPKASELRAELAAGLSLVDPHRSWHTDRSPVLRIA
DWLTRACTSLSKLGTDCLALRQSGIDELTTITAGASSTMPQKQNPVAASALIALASQATA
QLSALHHAAAHQHQRDGASWFGEWLSLPQIVFCAASAARTAVSMTKGLAPDAKQMLRNLH
AANGTIYAEALSFALAEQMRRGEAQAAVKDLCVKAAQQGVPLQEVAALQFPELDHATLFD
ATRQLGSATYEAQRFVKIVAALKGRKEST