Protein Info for Psest_0445 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 890 PF13185: GAF_2" amino acids 187 to 326 (140 residues), 39.5 bits, see alignment E=2.9e-13 PF01590: GAF" amino acids 188 to 326 (139 residues), 65.6 bits, see alignment E=3.1e-21 TIGR00229: PAS domain S-box protein" amino acids 339 to 458 (120 residues), 52.7 bits, see alignment E=4.5e-18 PF00989: PAS" amino acids 340 to 450 (111 residues), 37.1 bits, see alignment E=1.2e-12 PF08448: PAS_4" amino acids 345 to 453 (109 residues), 40.4 bits, see alignment E=1.4e-13 PF13426: PAS_9" amino acids 351 to 451 (101 residues), 56.8 bits, see alignment E=1.1e-18 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 460 to 621 (162 residues), 126 bits, see alignment E=1.2e-40 PF00990: GGDEF" amino acids 464 to 618 (155 residues), 130.6 bits, see alignment E=2.1e-41 PF00563: EAL" amino acids 638 to 873 (236 residues), 260.1 bits, see alignment E=8e-81

Best Hits

KEGG orthology group: None (inferred from 90% identity to psa:PST_3855)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH16 at UniProt or InterPro

Protein Sequence (890 amino acids)

>Psest_0445 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MKIHTDAASRLATEVVTQLPVPSRLGMLRFERLNESSWTLLFLDPACERLLGVSASDLCS
LIDSPYASLMEPSARLQLHEDIQQQLSARNHYSVSYRLHTPDSVLDITELGEVCQQYGRE
LLRGYLMAGRRPESDQDLRSQNARLRTSLQQHQQTQDEHLEHMLRSRTQQGLIVKLARHR
YSSTNPQQEAAQLITQAACEVYEVDRAAIWHLNGTRLEPIACYRLGSDNYETLPEVELTP
FPRYLQALQSGRAVDAQDVSQDHRTSALGEQLHRPQGIRSILDASIRVGGEVIGVLSLQH
AGQPRTWQADEVAFAGELADQYAQVLANQQRLSATHMLSLFQRAVEQSASAFILVDRDGR
VEYVNPAFTSISQFSAAEVRGHRLTDLKALENLSELLFDTSSVLAHSNSWQGEFRSRRKN
LEPYWGQLSISKVFNDEGVLTHYIGIYEDITRSKLAHQHIERLAYTDNLTNLGNRPYFIR
SLEERFAAGAEPRLCLLLVDIDNFKRINDSLGHQTGDKLLTSLARRLRNGLSQQSVLARF
ASNEFALLFDDMELDEGLRLADQVLRVLDKPLFVDKQLISVSASLGLACSPQHGRDPETL
MKHAGQALHKAKANGKNQVQIFTEALHAEANYKLFVENNLRRALTQNELEVFYQPKLCLR
SGQLQGLEALLRWNHPEKGMIRPDQFISVAEETGLIIPIGKWVLREACRMGVRLSALGHG
KPQIAINLSPKQFSDPDLLGSIASILLEEQLSPGQLELELTESLLLDATEETRQQLIGLK
ALGLTLAMDDFGTGYSSLSYLKKFPIDVIKIDRSFIKDIPDNQDDMEITSAVIAMARKLH
LKVVAEGIETAEQLKFLRHHRCDIGQGYLFDKPIPGNILSDALRRYPHWR