Protein Info for Psest_4372 in Pseudomonas stutzeri RCH2

Annotation: NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 769 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details transmembrane" amino acids 25 to 43 (19 residues), see Phobius details amino acids 51 to 70 (20 residues), see Phobius details amino acids 76 to 97 (22 residues), see Phobius details amino acids 106 to 124 (19 residues), see Phobius details amino acids 130 to 149 (20 residues), see Phobius details amino acids 161 to 188 (28 residues), see Phobius details amino acids 200 to 219 (20 residues), see Phobius details amino acids 239 to 258 (20 residues), see Phobius details amino acids 268 to 287 (20 residues), see Phobius details amino acids 298 to 316 (19 residues), see Phobius details amino acids 322 to 346 (25 residues), see Phobius details amino acids 365 to 386 (22 residues), see Phobius details amino acids 404 to 426 (23 residues), see Phobius details amino acids 446 to 465 (20 residues), see Phobius details amino acids 495 to 516 (22 residues), see Phobius details amino acids 564 to 584 (21 residues), see Phobius details amino acids 596 to 614 (19 residues), see Phobius details amino acids 621 to 640 (20 residues), see Phobius details amino acids 646 to 666 (21 residues), see Phobius details amino acids 686 to 706 (21 residues), see Phobius details amino acids 741 to 761 (21 residues), see Phobius details PF00662: Proton_antipo_N" amino acids 63 to 103 (41 residues), 31.2 bits, see alignment 3.3e-11 PF00361: Proton_antipo_M" amino acids 126 to 411 (286 residues), 207.1 bits, see alignment E=7.2e-65 PF13244: MbhD" amino acids 605 to 669 (65 residues), 62.3 bits, see alignment E=8e-21 PF20501: MbhE" amino acids 679 to 761 (83 residues), 64.9 bits, see alignment E=1.2e-21

Best Hits

KEGG orthology group: K05565, multicomponent Na+:H+ antiporter subunit A (inferred from 92% identity to psa:PST_4209)

Predicted SEED Role

"Na(+) H(+) antiporter subunit A" in subsystem Sodium Hydrogen Antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTY9 at UniProt or InterPro

Protein Sequence (769 amino acids)

>Psest_4372 NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit (Pseudomonas stutzeri RCH2)
MLAALLVSFLGAILARFIVSRTFPHGGWVAALLPAGLFLFFLAKGASLPEVGAITETLEW
VPALGISLTLRLDGFALLFVLLITGIGTLVTIYAGAYFSHSPKEEAARFLTLILLFMTAM
LGTVLSDNLIVMFVFWEATSLLSFMLIGFNSHRAQARKAALQSLIVTGGGGLALFGGILL
IGITLGTFSLSEVAQRAPELLASPLAVPAMILIMLGAFTKSAQLPFHFWLPQAMEAPAPA
SAFLHSATMVKLGVYLLARFDLIFEGIPAFGSTLVIVGSLTMLVAAVRALSTDGFKEVLA
HSTVGSLGVLVMLIGLDGDYSVTALIAFIIAHALYKAALFFCAGTTIHAVGEGRLSKIGG
LARRLPMTTLAAGMAAISMAGLPPTLGFITKEYLFESQLNASSGWVVVAVAVLVNAVFAA
IAGVAAIRPYYLGKTRSEIKHPETLGLYLGPLVLGGLGFLFGLAPDFLVRGLIQPANDVL
VGHTVDLSFSLWHGFTPMLALSATVVAFAGGLIAYWHRIHYALSLNKGLARVLGDFGFNA
AFAGLLRLAERCTRFLQNGDQHRYTSTVAAAVLLIIGYGVLASGTRPLIAMTGGRFDAPS
IIVLLLMCLGAFAATRTPSLLRAMIAVGVVGFGSALIFLLNGAPDVALTQFSVEVLLVLI
LVALLLRVPERAASTREPREKRLDILISAGFALVVFVALAATVALPLDGRISEFYAATSY
LEAHGRNVVNVVLVDYRAIDTLGEVVVVAFAAMSVWGLLRALPRRRAAP