Protein Info for Psest_4349 in Pseudomonas stutzeri RCH2

Annotation: glucosamine--fructose-6-phosphate aminotransferase (isomerizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 616 (615 residues), 860.5 bits, E=3.4e-263
• PF13230: GATase_4
  • amino acids 60 to 149 (90 residues), 25 bits, E=1.7e-09
• PF13522: GATase_6
  • amino acids 66 to 183 (118 residues), 69.2 bits, E=7.5e-23
• PF13537: GATase_7
  • amino acids 83 to 193 (111 residues), 54.9 bits, E=1.8e-18
• PF01380: SIS
  • amino acids 291 to 424 (134 residues), 107.5 bits, E=9.2e-35
  • amino acids 469 to 598 (130 residues), 86.1 bits, E=3.8e-28

Best Hits

Swiss-Prot: 88% identical to GLMS_PSESM: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 95% identity to psa:PST_4186)

MetaCyc: 63% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See L0GPY1 at UniProt or InterPro

Protein Sequence (616 amino acids)

>Psest_4349 glucosamine--fructose-6-phosphate aminotransferase (isomerizing) (Pseudomonas stutzeri RCH2)
MCGIVGAVAERNITAILLEGLKRLEYRGYDSAGVAVLDDSGTLERLRRNGKVAELEQAQQ
QTPLVGRLGIAHTRWATHGAPCERNAHPHFSGDELAVVHNGIIENHEALRAQLKDMGYVF
VSDTDTEVIVHLLHHKLESLGDLTAALKAAVKELHGAYGLAVISAKQPDRLLAARSGSPL
VIGLGLGENFLASDQLALRQVTDRFMYLEEGDIAEIRRDSVQIWDVDGEPVQREAVQYHE
GAEAADKGAYRHFMLKEIHEQPKVVQRTLEGRLGDRQVLVEAFGPQAGELFAKVRNVQIV
ACGTSYHAGMVARYWLEELAGIPCQVEVASEFRYRKVVVQPDTLFVTISQSGETADTLAA
LRNAKQRSTEEGGYLASLAICNVGISSLVRESDLTLLTQAGPEIGVASTKAFTTQLVGLM
LLTLSLGHVRGTLSAASEAELVEELRRLPTRLGEALAMDTTVEKISEHFAEKHHTLFLGR
GAQYPVAMEGALKLKEISYIHAEAYPAGELKHGPLALVDADMPVVTVAPNNELLEKLKSN
LQEVRARGGELIVFADREAGFDNAEGIFVVNMPHIHDALAPILYTLPLQLLSYYVAVLRG
TDVDQPRNLAKSVTVE