Protein Info for Psest_4310 in Pseudomonas stutzeri RCH2

Annotation: ABC-type Mn2+/Zn2+ transport systems, permease components

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 261 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 42 to 72 (31 residues), see Phobius details amino acids 84 to 104 (21 residues), see Phobius details amino acids 127 to 148 (22 residues), see Phobius details amino acids 161 to 180 (20 residues), see Phobius details amino acids 186 to 203 (18 residues), see Phobius details amino acids 214 to 234 (21 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details PF00950: ABC-3" amino acids 3 to 256 (254 residues), 257.3 bits, see alignment E=1.7e-80 PF01032: FecCD" amino acids 40 to 234 (195 residues), 28.7 bits, see alignment E=7e-11

Best Hits

Swiss-Prot: 60% identical to ZNUB_ECOLI: High-affinity zinc uptake system membrane protein ZnuB (znuB) from Escherichia coli (strain K12)

KEGG orthology group: K09816, zinc transport system permease protein (inferred from 98% identity to psa:PST_0041)

MetaCyc: 60% identical to Zn2+ ABC transporter membrane subunit (Escherichia coli K-12 substr. MG1655)
ABC-63-RXN [EC: 7.2.2.20]

Predicted SEED Role

"Zinc ABC transporter, inner membrane permease protein ZnuB" in subsystem Transport of Zinc

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.2.2.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GU01 at UniProt or InterPro

Protein Sequence (261 amino acids)

>Psest_4310 ABC-type Mn2+/Zn2+ transport systems, permease components (Pseudomonas stutzeri RCH2)
MPDFLLNALLAGLALALVAGPLGSFVVWRRMAYFGDTLSHAALFGVALGLMLDVNLTLAV
TVGCVLLALLLVTLQQRQPLASDTLLGILAHSTLSLGLVSLSFMKDVRVDLMGYLFGDLL
AVGPSDLAWIMGGSALVLLMLIPLWRPLLAITVHEELAKVEGLPVAGIRLALMLLIAVVI
AVAMKIVGVLLITSLLIIPAAAAQRHARTPEQMAFGASLLGIVAVCLGLTLSWYEDTPAG
PSIVVSAAALFLLSFAWPKRS