Protein Info for HP15_4173 in Marinobacter adhaerens HP15

Annotation: NADH dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 PF01512: Complex1_51K" amino acids 146 to 318 (173 residues), 145.9 bits, see alignment E=9.3e-47 PF10589: NADH_4Fe-4S" amino acids 430 to 511 (82 residues), 94.5 bits, see alignment E=2.8e-31

Best Hits

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 76% identity to hel:HELO_1898)

Predicted SEED Role

"NAD-dependent formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PMM9 at UniProt or InterPro

Protein Sequence (512 amino acids)

>HP15_4173 NADH dehydrogenase (quinone) (Marinobacter adhaerens HP15)
MLKRIYVPCDTTALSMGADKVAKLIERHANARGLEIELVRNGSRGLFWLEPLVEVETDSG
RIGFGPVEAHDVDALVDAGLFEGKSDHPLCLGMVEDISYLKLQQRLTFSRIGITDPLSIA
DYRSHGGFVGLEQAVSLSPQAIVDEVKASGLRGRGGAAFPTGIKWQTVHDEPWQQQKYIV
CNADEGDSGTFADRLAMECDPYMLIEGMTIAGLAVGATQGFIYLRSEYPVAHRILNEAIS
RAEAEGYLGRDVRGSGRAFNLEVRLAAGAYICGEETSLLESLEGKRGLVRAKPPLPAIKG
LFGQPTVVNNVLSFAAVPFILAKGGQTYAHYGMGKSRGTLPIQLAGNIRRGGLIELAFGV
SLREILEDFGGGTFTGRPMKAVQVGGPLMAYMPQNQWDTPMDYEAFAQLGAGIGHGGVVV
FDDTVDMGEQARFAMEFCTVESCGKCTPCRIGSVRGLEVIDRIRAGESPEANLVLLEELC
ETMVDGSLCAMGGMTPFPVQSVMKYFPDDLTA