Protein Info for Psest_4299 in Pseudomonas stutzeri RCH2

Annotation: diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 545 PF01627: Hpt" amino acids 18 to 89 (72 residues), 28.8 bits, see alignment E=1.7e-10 PF00072: Response_reg" amino acids 257 to 368 (112 residues), 75.3 bits, see alignment E=6.3e-25 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 383 to 543 (161 residues), 147.6 bits, see alignment E=1.4e-47 PF00990: GGDEF" amino acids 384 to 539 (156 residues), 143.4 bits, see alignment E=8.1e-46

Best Hits

KEGG orthology group: None (inferred from 84% identity to psa:PST_0052)

Predicted SEED Role

"Probable two-component response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPT1 at UniProt or InterPro

Protein Sequence (545 amino acids)

>Psest_4299 diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MDEQVRFALQEQLAQLEQQFGERLQGDLADLGTLATELQHNRAPGNRRQLMLGIRERLHR
LAGAAGTFGFAKLGERARQLEQRADRWLDSAKPGSRAVESFSRALQQLASQTPGQERAAA
ELPDHHDEPAPGCRIYLLEADPVAGCSMALTLRNFGYLVSQWQDFAALQQAVATEPPDAL
IVSVQHDSELESVASLQQGLDHPLPLLVIHERIDFTSQLAAVRAGAQGFFSRPLDITQLE
NSLERCLDRQQGEPFRVLIVDDDAELAARYSLVLRNSQMQVQTLTEPTQVLETMRSFNPE
VLLLDVNMPGCSGPELAQMIRLHDEWLRVTIIYLSAETDIQRQMAALLKAGDDFITKPIS
DTALVASVYSHAQRARSLSTALARDSLTGLLKHADIKEQLALEVQRATRSGKPASVVMLD
LDHFKHVNDTYGHAAGDNVLRALANLLRQRLRRIDSLGRYGGEEFVAVLPECTALQARRI
FDEIRVRFAALSFYGGDSKEFYVTFSAGICQTDGRTASGVLLERADQALYVAKHKGRNQV
QVAEH