Protein Info for Psest_4290 in Pseudomonas stutzeri RCH2

Annotation: N-carbamoylputrescine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details TIGR03381: N-carbamoylputrescine amidase" amino acids 5 to 282 (278 residues), 486.5 bits, see alignment E=1.2e-150 PF00795: CN_hydrolase" amino acids 6 to 266 (261 residues), 195 bits, see alignment E=7.5e-62

Best Hits

Swiss-Prot: 63% identical to AGUB_SOLLC: N-carbamoylputrescine amidase (CPA) from Solanum lycopersicum

KEGG orthology group: K12251, N-carbamoylputrescine amidase [EC: 3.5.1.53] (inferred from 96% identity to psa:PST_0061)

MetaCyc: 85% identical to N-carbamoylputrascine amidohydrolase subunit (Pseudomonas aeruginosa)
N-carbamoylputrescine amidase. [EC: 3.5.1.53]

Predicted SEED Role

"N-carbamoylputrescine amidase (3.5.1.53)" in subsystem Arginine and Ornithine Degradation or Polyamine Metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTY3 at UniProt or InterPro

Protein Sequence (293 amino acids)

>Psest_4290 N-carbamoylputrescine amidase (Pseudomonas stutzeri RCH2)
MSRVVTVAATQMACSWDRQANIANADKLVREAAAKGAQIILIQELFETPYFCQKPNAEYL
QLATPVEENPAIQHFQKVAAELQVVLPISFFELAGRARFNSIAIIDADGKLLGVYRKSHI
PDGPGYHEKYYFNPGDTGFKVWNTRYAKIGVAICWDQWFPETARSMALMGAELLFYPTAI
GSEPHDPNITSRDHWQRVQQGHAGANLMPLIASNRIGREEQDGYDITFYGSSFIADQFGA
KVEEMDETSEGVLVHQFDLDQLEHIRSAWGVFRDRRPNLYGSIRTLDGSQPSE