Protein Info for Psest_4288 in Pseudomonas stutzeri RCH2

Updated annotation (from data): Glutamate--putrescine ligase (EC 6.3.1.11)
Rationale: Specifically important for utilizing L-Arginine and Putrescine Dihydrochloride. Automated validation from mutant phenotype: the predicted function (RXN0-3901) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Glutamine synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 120 to 453 (334 residues), 365 bits, E=1.9e-113

Best Hits

Swiss-Prot: 45% identical to PUUA_ECOLI: Gamma-glutamylputrescine synthetase PuuA (puuA) from Escherichia coli (strain K12)

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 97% identity to psa:PST_0063)

MetaCyc: 45% identical to glutamate-putrescine ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--putrescine ligase. [EC: 6.3.1.11]

Predicted SEED Role

"Glutamine synthetase (EC 6.3.1.2)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.1.2)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-aspartate degradation II (aerobic) (2/3 steps found)
• L-aspartate degradation III (anaerobic) (2/3 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• ammonia assimilation cycle II (1/2 steps found)
• superpathway of L-arginine and L-ornithine degradation (8/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (6/11 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See L0GSI7 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Psest_4288 Glutamate--putrescine ligase (EC 6.3.1.11) (Pseudomonas stutzeri RCH2)
MAPPRAVQLNEANAFLKEHPEVQYVDLLITDMNGVVRGKRIERASLHKVYEKGINLPASL
FALDINGITVESSGLGMDIGDSDRTCFPIPNTLCKEPWQKRPTAQLLMTMHERDGDPFFA
DPREVLRQVVSKFDELGLTICAAFELEFYLIDQENINGRPQPPRSPISGKRPHSTQVYLI
DDLDEYVDCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPIKACDYAVLLKRLI
KNIAYDHEMDTTFMAKPYPGQAGNGLHVHISLLDKNGNNIFATENPLESAPLRHAIGGLQ
ETMAASMAFLCPNVNSYRRFGAQFYVPNAPCWGMDNRTVALRVPTDSPDAVRVEHRVAGA
DANPYLMLASILAGVHHGLTNKIEPDAPIEGNSYEQVEQSLPTNLRDALRELDDSEIMAR
YISPDYIDIFVACKESELAEFEHSISDLEYNWYLHTV