Protein Info for Psest_4269 in Pseudomonas stutzeri RCH2

Updated annotation (from data): C4-dicarboxylate transporter, DctQ subunit
Rationale: Important for succinate and fumarate utilization (KEGG_correct). The other components (Psest_4268 and Psest_4270) were already annotated correctly
Original annotation: TRAP-type C4-dicarboxylate transport system, small permease component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 208 transmembrane" amino acids 15 to 37 (23 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 78 to 102 (25 residues), see Phobius details amino acids 113 to 137 (25 residues), see Phobius details amino acids 158 to 176 (19 residues), see Phobius details PF04290: DctQ" amino acids 65 to 184 (120 residues), 76.6 bits, see alignment E=8.6e-26

Best Hits

Swiss-Prot: 74% identical to DCTQ_PSEAE: C4-dicarboxylate TRAP transporter small permease protein DctQ (dctQ) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11689, C4-dicarboxylate transporter, DctQ subunit (inferred from 97% identity to psa:PST_0077)

Predicted SEED Role

"TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter" in subsystem Sialic Acid Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPQ3 at UniProt or InterPro

Protein Sequence (208 amino acids)

>Psest_4269 C4-dicarboxylate transporter, DctQ subunit (Pseudomonas stutzeri RCH2)
MNALWRVWDHFEEGFIAFLLAAMTLVTFVYVILNNLYTLFYNLGDRFEGSADFWFAIGDF
IIGLAQAMTWSTALTKALFAWLIFSGLAYGVRTAGHIGVDALVKLAPRHIQRIIGIIACL
LCLGYAGLLTVASFEWIQTLFIANIGAEDLGHIGVKQWHIGMIVPFGFAMVFIRFAEIFV
RILRNEQTGLGLADEAADALKHGEEEPK