Protein Info for Psest_4265 in Pseudomonas stutzeri RCH2

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 285 (285 residues), 348.8 bits, E=4e-108
• TIGR01214: dTDP-4-dehydrorhamnose reductase
  • amino acids 2 to 284 (283 residues), 342.5 bits, E=8.4e-107
• PF01370: Epimerase
  • amino acids 3 to 155 (153 residues), 70.5 bits, E=3.1e-23
• PF16363: GDP_Man_Dehyd
  • amino acids 32 to 152 (121 residues), 45.5 bits, E=1.5e-15
• PF02719: Polysacc_synt_2
  • amino acids 33 to 139 (107 residues), 25 bits, E=2.1e-09

Best Hits

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 87% identity to psa:PST_0081)

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (7/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (14/33 steps found)

KEGG Metabolic Maps

• Polyketide sugar unit biosynthesis
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.133

Use Curated BLAST to search for 1.1.1.133

Sequence Analysis Tools

See L0GTW0 at UniProt or InterPro

Protein Sequence (302 amino acids)

>Psest_4265 dTDP-4-dehydrorhamnose reductase (Pseudomonas stutzeri RCH2)
MKILITGSQGQLARELQLELAGKLLALGHNALNLADPEQIREQVRLVRPDLIINAAAYTA
VDPAETHREQAFAVNARGPQVLAEEAARLGVPLIHYSTDYVFDGRKGEPYTEADVPQPLS
VYGASKLAGEQAIQAVGGEYLILRTSWVYSQHGKNFLLTMQRLLQERDALSVVSDEIGAP
TWAATIARTTAEMVRKRSAGEAGPSGLYHLTAGGETSWYGFACSIAEHLRQQGRLRAEIT
PILSKDYPTAAQRPLNSRLNCARLQQDWGIRLPDWETALHECLVQSDEQAVASAPRTATV
GR