Protein Info for Psest_4225 in Pseudomonas stutzeri RCH2
Annotation: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 97% identity to psa:PST_0109)Predicted SEED Role
"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
- superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
- superpathway of purine nucleotide salvage (11/14 steps found)
- adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
- guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
- guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.17.4.1
Use Curated BLAST to search for 1.17.4.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GTI4 at UniProt or InterPro
Protein Sequence (715 amino acids)
>Psest_4225 ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (Pseudomonas stutzeri RCH2) MAKTDGPIAADSKSTSIALQAASEDIWAQKYRLTSKDGTPIDQSVDDTWQRVARALADVE PAKQREHWYERFLWALRNGAIPAGRIISNAGAQDYKPATSTINCTVSGSITDSMDDILGK VHEAGLTLKAGCGIGYEFSTLRPRGSFVSGAGAHTSGPLSFMDIFDKMCFTVSSAGGRRG AQMGTFDVGHPDVREFIRAKREDGRLRQFNLSLLITDEFMQAVEADGEWPLIFPVHAKEA GELDLADAEHVLWREWPVHDGYIVRDDGLVACKIYGRVKARHLWDMIMVSTYDYAEPGFI LIDRVNQLNNNWWCEAIRATNPCGEQPLPPYGSCLLGSINLTNFVIDPFGADARFDWDKY REVVRIFTRMLDNVVEINGLPLEQQRHEIESKRRHGMGFLGLGSTLTLLKLRYGSPEACV FTEEVAREMALVGWEQALELSKEKGAAPLLSQTFEVTAEMLRKRPEMAKDGYKVGDQIAG RVLHAKYSRYMQKIAEYAPELIEALAEQGARFTHHSSIAPTGTISLSLANNASNGIEPSF AHHYSRNLIRPGRKAKEKIEVFSYELLAYRTLINERAKPGSDEPGEKLPDYFITADDVSP TQHVDIQAAAQKWVDSSISKTANVPTDYPFEAFKDIYRYAWRQGLKGCTTFRFNPAAFQG VLVKESDLEKTLYRFTLEDGSVVELKGNQEVEYDGEVNTAANLFDALKEGYYGKY