Protein Info for Psest_4223 in Pseudomonas stutzeri RCH2

Annotation: Predicted membrane-associated, metal-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 290 transmembrane" amino acids 9 to 32 (24 residues), see Phobius details amino acids 38 to 58 (21 residues), see Phobius details amino acids 70 to 90 (21 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details amino acids 149 to 168 (20 residues), see Phobius details amino acids 180 to 199 (20 residues), see Phobius details amino acids 207 to 228 (22 residues), see Phobius details amino acids 236 to 258 (23 residues), see Phobius details amino acids 264 to 284 (21 residues), see Phobius details PF00892: EamA" amino acids 16 to 138 (123 residues), 37.6 bits, see alignment E=1.2e-13 amino acids 150 to 281 (132 residues), 53 bits, see alignment E=2.1e-18

Best Hits

KEGG orthology group: None (inferred from 64% identity to axy:AXYL_03456)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPL6 at UniProt or InterPro

Protein Sequence (290 amino acids)

>Psest_4223 Predicted membrane-associated, metal-dependent hydrolase (Pseudomonas stutzeri RCH2)
MTFNKSLWTSYGVMTVFVLLWGSAAIFTRWGLDHGTPFALLIVRFALALTAVLLIGLYRR
RWRPAPGTAWQTAATGLLMIGCYSICYFQAMAHGVTPGVIATLLGVQPILTLLLLERRFS
PLRLLGLLVALAGLAAVVFQSLVLVRFSLAGMLFALGALLCMTVGAILQQRVRQSPLEVM
PLQYAVSLLLCLAFVPLQPIQLEAVTGFIIPVLWLGLVISVAAQLLLYRMIQTGNLVNVT
SLFYLVPVVTVGMDYLFLGNQLSRIGLLGMGAILLGLALVFRAAPQPHRG