Protein Info for Psest_4222 in Pseudomonas stutzeri RCH2

Annotation: Predicted Rossmann fold nucleotide-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF14793: DUF4478
  • amino acids 10 to 116 (107 residues), 144.2 bits, E=2.1e-46
• PF03641: Lysine_decarbox
  • amino acids 206 to 331 (126 residues), 86.8 bits, E=2.2e-28
• PF11892: PpnN_C
  • amino acids 336 to 456 (121 residues), 183.3 bits, E=2.4e-58

Best Hits

Swiss-Prot: 56% identical to PPNN_ECOLI: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli (strain K12)

KEGG orthology group: K06966, (no description) (inferred from 97% identity to psa:PST_0111)

MetaCyc: 56% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

• adenosine nucleotides degradation III (1/1 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

See L0GSC2 at UniProt or InterPro

Protein Sequence (457 amino acids)

>Psest_4222 Predicted Rossmann fold nucleotide-binding protein (Pseudomonas stutzeri RCH2)
MTQRNVINASVSPKGSLETLSQREVQQLSEVGSGSTHKLFRQCALAILNTGTRIDNAKTI
LEAYEDFEVRILQQDRGVRLELFNAPADAFVDGEMIASTREMLFSALRDIVYTESELSSA
RIDLSTSQGITDYVFHLLRNARTLRPGIEPNMVVCWGGHSISTEEYKYSKRVGHELGLRS
LDVCTGCGPGVMKGPMKGATIAHAKQRRNGSRYLGLTEPGIIAAEAPNPIVNELVILPDI
EKRLEAFVRVGHGIIIFPGGVGTAEEFLYLLGILLHPANCDVPFPVILTGPSDAADYLQQ
LHDFVGATLGEEAQKRYQIVFNDPTEVARQMTEGLREVRRFRRERNDAFHFNWLLKIEEG
FQRPFDPTHEAMASLPLRRDLPPHELAANLRRAFSGIVAGNVKDKGIRRIEQYGRYEIHG
DAAIMQPLDKLLQAFVEQHRMKLPGGVPYTPCYRVVS