Protein Info for Psest_4219 in Pseudomonas stutzeri RCH2

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 914 PF02739: 5_3_exonuc_N" amino acids 6 to 167 (162 residues), 181.8 bits, see alignment E=2.1e-57 TIGR00593: DNA polymerase I" amino acids 6 to 914 (909 residues), 932.4 bits, see alignment E=1.7e-284 PF01367: 5_3_exonuc" amino acids 168 to 270 (103 residues), 94.6 bits, see alignment E=1.1e-30 PF01612: DNA_pol_A_exo1" amino acids 315 to 501 (187 residues), 121.8 bits, see alignment E=7e-39 PF22619: DNA_polI_exo1" amino acids 379 to 469 (91 residues), 44.7 bits, see alignment E=3e-15 PF00476: DNA_pol_A" amino acids 536 to 912 (377 residues), 547.7 bits, see alignment E=3.6e-168

Best Hits

Swiss-Prot: 82% identical to DPO1_PSEAE: DNA polymerase I (polA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 80% identity to avn:Avin_00630)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTR6 at UniProt or InterPro

Protein Sequence (914 amino acids)

>Psest_4219 DNA polymerase I (Pseudomonas stutzeri RCH2)
MSQAPLILVDGSSYLYRAFHALPPLTTSTGKPTGAVKGVLNMLLSLRRQYPDSPFAVVFD
AKGPTFRDALFENYKSHRPPMPDDLRSQVEPLHASVRALGMPLLCVEGVEADDVIGTLAR
QCAALGRDVVISTGDKDMAQLVCPHVTLVNTMTGSVYDIEGVKTKFGVGPELIIDFLALM
GDKVDNIPGVPGVGEKTACGLLNGIPGGLKGLYDNLDQVAGLPIRGAKSLGAKLAEHRDA
AFMSYELATIKIDVPLDVEVGDLMPGEPHREALIALYRELEFKNWLDDLLREAKAAGENC
EVQPEGCSIQAEAQYETLLEQADFERWLDRLKTAECFAFDTETTSIDAQRAELVGVSFAI
EPGQAAYVPLRHSYMGVPQQLELDAVLAALKPLLEDPAKTKICQHGKYDMNVLMHYGIEM
RGMTFDTMLESYVLDATATRHDMDSLALKYLGRGTIRFEDIAGKGAKQLTFDQIAIEQAG
PYAAEDADVTLRLHQTLLGKLEQTPSLLKVLTEIEMPLVPVLARIERNGALVDAQLLGQQ
SVELGDKLVQLEREAFEIAGEEFNLGSPKQLCAILYDKLGCPVISKTAGGQPSTAESVLA
ELAEQDYPLPKVIMQHRSLSKLKGTYTDKLPQQINPRTGRIHTSYHQAVTATGRLSSSDP
NLQNIPIRTAEGRRIRQAFVAAPGYKLLAADYSQIELRIMAHLAQDAGLLHAFQNDLDVH
RATAAEVFGVPLEQVSNDQRRSAKAINFGLIYGMSAFGLAKQIDVGRKEAQEYIDRYFAR
YPGVLAYMERTRTQAAEQGYVETLFGRRLYLPEINSKNGAMRKGAERTAINAPMQGTAAD
IIKRAMIAVDGWLQDSGLDARVILQVHDELVLEVREDLVEQVREAICPLMSGAAQLDVPL
LVEAGVGNNWDEAH