Protein Info for Psest_4200 in Pseudomonas stutzeri RCH2

Annotation: general secretion pathway protein G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 146 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details PF07963: N_methyl" amino acids 7 to 32 (26 residues), 35.3 bits, see alignment 6e-13 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 10 to 33 (24 residues), 31.1 bits, see alignment 1.4e-11 TIGR01710: type II secretion system protein G" amino acids 12 to 145 (134 residues), 214.8 bits, see alignment E=3.9e-68 PF08334: T2SSG" amino acids 35 to 144 (110 residues), 149.6 bits, see alignment E=3.1e-48

Best Hits

Swiss-Prot: 87% identical to GSPG_PSEAE: Type II secretion system protein G (xcpT) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02456, general secretion pathway protein G (inferred from 97% identity to psa:PST_0127)

MetaCyc: 55% identical to type II secretion system protein GspG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"General secretion pathway protein G"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTF6 at UniProt or InterPro

Protein Sequence (146 amino acids)

>Psest_4200 general secretion pathway protein G (Pseudomonas stutzeri RCH2)
MTMKLQRRTQGGFTLIEIMVVVVILGILAALVVPQVMSRPDQAKVTVAKGDIKAIGAALD
MYKLDNFTYPSTQQGLDALVKKPSGNPQPKNWNRDGYLKRLPKDPWGNDYQYLSPGTQGQ
FDLYSFGADGKPGGSDLNADIGNWDL