Protein Info for Psest_4197 in Pseudomonas stutzeri RCH2

Annotation: general secretion pathway protein J

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 237 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details PF07963: N_methyl" amino acids 6 to 31 (26 residues), 29.2 bits, see alignment (E = 4.9e-11) TIGR01711: type II secretion system protein J" amino acids 9 to 209 (201 residues), 200.5 bits, see alignment E=2.4e-63 TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 9 to 30 (22 residues), 24.5 bits, see alignment (E = 1.7e-09) PF11612: T2SSJ" amino acids 61 to 206 (146 residues), 144.2 bits, see alignment E=3.4e-46

Best Hits

Swiss-Prot: 52% identical to GSPJ_PSEAE: Type II secretion system protein J (xcpW) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02459, general secretion pathway protein J (inferred from 95% identity to psa:PST_0130)

Predicted SEED Role

"General secretion pathway protein J"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GS98 at UniProt or InterPro

Protein Sequence (237 amino acids)

>Psest_4197 general secretion pathway protein J (Pseudomonas stutzeri RCH2)
MNAHARTSAGFTLLELLIAIALFALLGLGTYRMLEAVLQSDEAVRAQELQLRELSRAVWA
FERDLQQVVGRPIRDGFGDERNAFIGQLGGDDGAPVLELTRAGWRNPTGLRRANLQRVRW
RLAGQNLERVYWVVLDRDLDSEPRVQRVLENVQGLQLRYLDEEAVWHEEWPPFDFGRGSP
EDAAERLPVGLELSIEHARYGRVTRVLRLPDAAEQTTQQALPGAEGVPEPFEGELPQ