Protein Info for Psest_4174 in Pseudomonas stutzeri RCH2

Annotation: Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 470 PF00158: Sigma54_activat" amino acids 154 to 316 (163 residues), 227.3 bits, see alignment E=2.4e-71 PF14532: Sigma54_activ_2" amino acids 166 to 321 (156 residues), 72.6 bits, see alignment E=1e-23 PF07728: AAA_5" amino acids 174 to 293 (120 residues), 30.4 bits, see alignment E=9e-11 PF02954: HTH_8" amino acids 425 to 459 (35 residues), 29.5 bits, see alignment 1.3e-10

Best Hits

KEGG orthology group: None (inferred from 69% identity to pfv:Psefu_3042)

Predicted SEED Role

"Flagellar regulatory protein FleQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTM5 at UniProt or InterPro

Protein Sequence (470 amino acids)

>Psest_4174 Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains (Pseudomonas stutzeri RCH2)
MVTLKSISQAMNYSCDELFDELPAKKIAIIGSAHEAACDFLQVGLRRQGCKVERYDDLDG
LVKAAIDQFSLIFVVVGSLPARSLYAQVEALTRRARNVSVIPVVEYADQEKAAALLEIGC
VDYLLSPFSEAQLAALLRRQVCAETAQESFVSCSQAGRRLLAMAQRVSLTRAPILITGET
GTGKELMARYIHRFSASPDAPFIAVNCAAIPEQMLESILFGHEKGAFTGAVSAQPGKFEL
ANGGTLLLDEIGELPLGLQAKLLRVLQEQRVERLGGRREIELNVRIIAATNRDLQQEVAE
GRFRADLMFRLDVLPLHISPLRERKEDVLPLARRFIGKYAPQEAHDELLTEDACRALLQH
DWPGNARELENTVQRALVLRNGLFIQPQDLGLAAPAAASVRVEKPLTLAAENGKAALRAS
GKWAEYQHVIDTIRRFDGHKTKAAASLGMTSRALRYRLNAMREQGIELNF