Protein Info for Psest_4172 in Pseudomonas stutzeri RCH2

Annotation: flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 558 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 430 to 449 (20 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 25 to 550 (526 residues), 217.4 bits, see alignment E=2.2e-68 PF01514: YscJ_FliF" amino acids 48 to 219 (172 residues), 185.8 bits, see alignment E=6.8e-59 PF08345: YscJ_FliF_C" amino acids 250 to 410 (161 residues), 105.1 bits, see alignment E=4.9e-34

Best Hits

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 72% identity to pmy:Pmen_4417)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GS73 at UniProt or InterPro

Protein Sequence (558 amino acids)

>Psest_4172 flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF) (Pseudomonas stutzeri RCH2)
MLQKIKSRLPAGGLQLDPRVTLAGMAVIAAALAVAVAFYLWRDNGSFRPLHGAGESFPAA
EVMQILDGEALQYRIHPQSGQILVREDQLAQARLLLNAKGVKVAQPAGYELFDKEEPLGT
SQFVQDVRLKRSLEGELARTVMALKGVQQARVHLAQEENSSFVVSKRAPSKASVMLQLEP
GYKLSSDQVGAIVNLVANSVPNLKPEDVGVVDQYGALLSRGLNVGGGPAQNWQAVEDYQQ
KAAGNIEQVLAPVLGLGNFRISVAADIDFSQKEETFQSYGDTPRLRNEVLRNESALDRLA
LGVPGSLSNRPLPPEPDGEEAQQLATENKGATSLREESTRQMDYDQSVVHVKHAGFALRQ
QSVAVVLNSAAAPKGGWTDEARAEMEAMVRNAVGFKQERGDLLSLSVLPFAAVEQIEQVV
PWWENSQIHALAKVGVAGLIALLLLLIVVRPAVRNLTQRNVQALPQGEGLEGSLVEPSAP
AALEGDARPALASPRESNGPHIFGELNPLSEIRLPAPGSGLELQIEHLQMLAKNDPERVS
EVIKHWIGRNERDLNPAG