Protein Info for Psest_4133 in Pseudomonas stutzeri RCH2

Annotation: RNA polymerase sigma factor, sigma-70 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 13 to 167 (155 residues), 49.1 bits, see alignment E=2.4e-17 PF04542: Sigma70_r2" amino acids 16 to 82 (67 residues), 41.7 bits, see alignment E=1.2e-14 PF08281: Sigma70_r4_2" amino acids 114 to 165 (52 residues), 39.1 bits, see alignment 7.1e-14 PF20239: DUF6596" amino acids 183 to 282 (100 residues), 127 bits, see alignment E=5e-41

Best Hits

Swiss-Prot: 45% identical to Y370_RHIME: Uncharacterized protein R00370 (R00370) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 92% identity to psa:PST_0150)

Predicted SEED Role

"RNA polymerase sigma-70 factor, ECF subfamily"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPD1 at UniProt or InterPro

Protein Sequence (413 amino acids)

>Psest_4133 RNA polymerase sigma factor, sigma-70 family (Pseudomonas stutzeri RCH2)
MPLTGHFTAARVEAVYRSESRRVLATLIRLLGDFDRAEEALHDAFAAALQQWPRDGVPDN
PRAWLVSTGRFKAIDALRRRARFDASLRLLAEQLEDAAEAAEVDEMEDDRLRLIFTCCHP
ALPADGRVALTLREVCDLTTEEIARAFLAPPATIAQRIVRAKAKIRDARLPYRVPERDEL
PARLESVLRVVYLVFNEGYTASAGGELTRADLCAEAIRLGRLLLELLPNAEVMGLLALML
LHDARRAARTDAAGELVRLDEQDRSLWNRQQIAEGAQLVQLALTSRAFGAYALQAAIVAV
HAEAPSAAQTDWAQIVRLYDVLLQLSPSPVVELNRAVAVAMRDGAEAGLLLVDQLLERGD
LQDYHLSHATRADLLRRLARTDEAREAYRRALRLVQLEPERRFLQRQLASLGG