Protein Info for Psest_4104 in Pseudomonas stutzeri RCH2

Annotation: formate dehydrogenase, alpha subunit, proteobacterial-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1024 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 3 to 1023 (1021 residues), 1489 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 45 to 105 (61 residues), 58.2 bits, see alignment 9.9e-20 PF00384: Molybdopterin" amino acids 108 to 592 (485 residues), 104.8 bits, see alignment E=7.9e-34 PF01568: Molydop_binding" amino acids 901 to 1017 (117 residues), 49.7 bits, see alignment E=5.1e-17

Best Hits

Swiss-Prot: 68% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 72% identity to bav:BAV3142)

MetaCyc: 68% identical to formate dehydrogenase O subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTG0 at UniProt or InterPro

Protein Sequence (1024 amino acids)

>Psest_4104 formate dehydrogenase, alpha subunit, proteobacterial-type (Pseudomonas stutzeri RCH2)
MDMNRRQFFKVCGVGLGASSMAALGMAPPTAYAESIRHFKLTNTRETRNTCPYCSVGCGL
ILYSQGSGGKNVAQNIIHIEGDSDHPVNRGTLCPKGAGLLDFVHSPNRLTHPEVREAGTN
EWKRIEWSEALDRIARLMKDDRDANFIERNDKGQTVNRWLTTGFLAASASSNEAGYITHK
VVRSLGILAFDNQARVUHGPTVASLAPTFGRGAMTNHWSDIQNADLVLIMGGNAAEAHPC
GFKWVTEAKARNKARLVVVDPRFTRSASVADMYAPIRTGTDIAFLGGLINYLLENDKIQH
EYVVNYTDVSFIVKEGFGFEDGLFNGYDAEKRSYPDKSAWSYELDEDGFARVDKSLTHPR
CVFNLLKQHYSRYTPEVVSNICGTPQDKMLQVWETIAETSAPGKVMTIMYALGWTQHSVG
SQMIRTGAMVQLLLGNIGMPGGGMNALRGHSNIQGLTDLGLLSNLLPGYMTLPLEAEQDY
TAYITKRTAKPLRPGQLSYWQHYEKFHVSLMKAWYGRNATAENNWGYDWLPKLDIPGYDV
LKVFDMMYQGQVNGYFCQGFNPIASFPNKAKVSAALAKLKYLVIMDPLATETSEFWRNAG
EYNDVDTASIQTTVFRLPTTCFAEEDGSLVNSGRWLQWHWKAAEPPGQAQTDVVIMGGLF
HRLRELYRKEGGAYAEPLLNIDWGYRQPEEPSAEEIAQEYNGKALSDVFDLQTGAQVAKA
GELLPGFGLLRADGTTSSGCWIFCGSWTQAGNQMARRDNADPYGMGQTLGWAWAWPANRR
ILYNRASADPAGNPWDKQKKRLVWWDGGKWGGTDVPDFKVDSRPEDGMNPFIMNPEGVAR
LFAVDKMNEGPFPEHYEPFETPIGRNPLHPGNALAISNPAARVFKNDMELFGTAEDFPYA
ATTYRLTEHFHFWTKHCRLNAITQPEQFVEIGEVLAKELGIVAGERVKVSSNRGYIKAVA
VVTKRIKPLTVDGQTVHQIGIPLHWGFAGVARNGYLTNTLTPFVGDANTQTPEFKSFLVN
VEKA