Protein Info for Psest_4097 in Pseudomonas stutzeri RCH2

Annotation: PAS domain S-box/diguanylate cyclase (GGDEF) domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 transmembrane" amino acids 39 to 57 (19 residues), see Phobius details amino acids 63 to 80 (18 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details amino acids 130 to 170 (41 residues), see Phobius details amino acids 179 to 199 (21 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 274 to 399 (126 residues), 26.6 bits, see alignment E=5.4e-10 amino acids 400 to 523 (124 residues), 69 bits, see alignment E=4.3e-23 PF08447: PAS_3" amino acids 322 to 387 (66 residues), 47.8 bits, see alignment 4.9e-16 amino acids 425 to 509 (85 residues), 27.7 bits, see alignment E=9.4e-10 PF13188: PAS_8" amino acids 405 to 451 (47 residues), 29.7 bits, see alignment 1.6e-10 PF00989: PAS" amino acids 407 to 512 (106 residues), 43 bits, see alignment E=1.4e-14 PF08448: PAS_4" amino acids 408 to 517 (110 residues), 38.7 bits, see alignment E=3.7e-13 PF13426: PAS_9" amino acids 413 to 514 (102 residues), 56.4 bits, see alignment E=1.1e-18 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 525 to 686 (162 residues), 117.8 bits, see alignment E=3.9e-38 PF00990: GGDEF" amino acids 528 to 683 (156 residues), 147.7 bits, see alignment E=9.1e-47 PF00563: EAL" amino acids 704 to 939 (236 residues), 266.2 bits, see alignment E=8.3e-83

Best Hits

KEGG orthology group: None (inferred from 87% identity to psa:PST_0174)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GS01 at UniProt or InterPro

Protein Sequence (950 amino acids)

>Psest_4097 PAS domain S-box/diguanylate cyclase (GGDEF) domain (Pseudomonas stutzeri RCH2)
MTDSIRAACPASAQPLPDAQALCDQTAERTRLLYRGSRIPAVLLLLTTLVCGVVLWGEQA
GVELGVWLVWMLGLGLLRLQQVMAFNRASPELQAAPHWRWRFMLGSAASALSLCYAMVAL
VPADAFVTQALLYGLIGSVVVAASVAYGVSLPAFFSFAVPSLLPTGLLLINSGHPLQQGW
GLFALIVLATLSLIAWQISRLLSDGLEQRWDKLQLIERLEKVGRDSERLNVRLANEVEQR
RRAEQQLQRAYDGLEQRVVERTAELARAAEELGESEARLNLALDASGLGLWDWDLQHNRV
HHSRLEVVFGVGLEKRHAEDGSLLPDIHPQDLPIVHATLIAHLKGESEQFAVEYRAVRAD
GSEICIEDRGRVIQRDASGRALRMIGTRRDISDVRRQAEQQRLAATVFEAASEGMVIMDA
TYRVLAVNDACCALSGYEREELLGRSIARIAGTPESQRQYEAMRDALERHGHWQGELIEK
RKSGEVYPQWVQLREVRDSDDSVTHVVAFISDLSVRRKIEERLRYLTHYDDLTGLANRGL
FKERLHEACQRVRRNGRNMAVLYIDLDRFKLLNESLGHEAADALLREVSRRLSTTLAEAD
TIARLSGDEFVVLLDAYGSLSSLAHLGSRLLQRIRKPMVIDEQELVISASIGVSLLPDNS
RDAEALLRQANIAMQQAKHLGGNTLQFFTDRPQASSMHYLQLENQLRKALEVGQLEVFYQ
PRLSLADDSLEAAEALVRWRHPELGLVAPAQFIPLAEETGLIIPLGEFVLREACRQARQW
QQDGLAAIRVSVNLSVKQLRQGNFVSLVRQVLEETGLPATMLELELTESQLLDDIDNAIS
ISEQLRALGVKLAIDDFGTGFSSLSYLKRFPVDYVKIDRSFISELEHSSQDAAITRAIIA
MVHSLERKVVAEGVETQAQMDFLKANHCDEIQGYLLSPPVPALQFAELLR