Protein Info for Psest_4072 in Pseudomonas stutzeri RCH2

Annotation: Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 910 PF00501: AMP-binding" amino acids 37 to 391 (355 residues), 191.1 bits, see alignment E=5.5e-60 PF13193: AMP-binding_C" amino acids 440 to 515 (76 residues), 58.3 bits, see alignment E=2.5e-19 PF00550: PP-binding" amino acids 547 to 611 (65 residues), 51.9 bits, see alignment 1.5e-17 PF00975: Thioesterase" amino acids 633 to 739 (107 residues), 67.1 bits, see alignment E=5.4e-22

Best Hits

KEGG orthology group: None (inferred from 87% identity to psa:PST_0199)

Predicted SEED Role

"O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26)" in subsystem Menaquinone and Phylloquinone Biosynthesis (EC 6.2.1.26)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.26

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRX8 at UniProt or InterPro

Protein Sequence (910 amino acids)

>Psest_4072 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II (Pseudomonas stutzeri RCH2)
MNHDSDATVPATDAVFAIPDPATGSIAPVTLHAALTRIAQGAPDRLAVLSTRFAPLDHRG
LQQMIEQTRRQLRLAGFGRDARIGVLLPEAPQAAVAIIAIACSAVAVPLDPRLGPAELDQ
FLKQLPLDALLIGSDGDQQGRAAAERHGLTLISAEAAEDGTPALLLDMPVAAQPAADELP
LPDAPAFILRSSGTTALPKLIPFTHRNMLTAARKWQRWFGLDAGDRCLCVSAPYYSHGLK
VTILTPLLAGGSVAFPLSPAVVDLHEWFEALRPSWYSAGPALHRAVLEAATTHPEGLGAH
RPRFASSGGAPLGQDIIDAFEQAMGFPLLEHYGSSEAAQIAVNTPGERKNGSVGRPWPET
LSIVDEDGLPVPTGQRGEIRVRGATVMPGYLGDPALNEVLREGWFHTGDIGSLDEHGFLY
LHGRLREVINRGGEKVSLPEVDAALLRHPAVADAAAFAVPHPRLGQDVAAAVVLHPQMSV
SPSELQNFLRGELVYFKVPRRVQVVDALPRGLTGKVLRHHLADAYVEQRSERARQAEASE
AASPLEQQVLALWRRLLKTEAVGPQDNFLDCGGDSLLATEMLTELEQMLGRVIPESVLVE
AETARELAERLENLAAHVIPVIDFNAQPGRTALFWFHGDFAHGGYYIRRLARLLGSQQPL
TAIAPHGMGDEPIPESVQQMARERLPLILERQPEGPYRIGGYCNGALVAFEAARLLVEAG
HRVEIVALIDPPTANVRPWSRAILRTLDRVLPDSYLARAYEALSRFGETSKWPYPKRLAN
LAWKLTHRGTRMVQQRLAGEVAPPPTARDREVRVRFLQYSLVMARYLPQRIDAPVVYFSA
DYTSRAWRNMGASFESYDVLGGHHRCVKDYTASIATPLRALLEDSAAPMPAQSGLRLPLT
DPVKRDGLVP