Protein Info for Psest_4071 in Pseudomonas stutzeri RCH2

Annotation: 4-hydroxyphenylpyruvate dioxygenase and related hemolysins

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 641 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01261: AP_endonuc_2" amino acids 18 to 262 (245 residues), 146.7 bits, see alignment E=2.1e-46 PF14696: Glyoxalase_5" amino acids 295 to 427 (133 residues), 116.3 bits, see alignment E=2.6e-37 PF00903: Glyoxalase" amino acids 441 to 562 (122 residues), 40.7 bits, see alignment E=5.9e-14

Best Hits

KEGG orthology group: K00457, 4-hydroxyphenylpyruvate dioxygenase [EC: 1.13.11.27] (inferred from 92% identity to psa:PST_0200)

Predicted SEED Role

"4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27)" in subsystem Aromatic amino acid degradation or Homogentisate pathway of aromatic compound degradation or Plastoquinone Biosynthesis or Tocopherol Biosynthesis (EC 1.13.11.27)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.27

Use Curated BLAST to search for 1.13.11.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRC6 at UniProt or InterPro

Protein Sequence (641 amino acids)

>Psest_4071 4-hydroxyphenylpyruvate dioxygenase and related hemolysins (Pseudomonas stutzeri RCH2)
MKRSIATVSLSGTLPEKLEAAAAAGFDGIELFENDLLYHSGSPLDVRRRCEELGLAVTLF
QPFRDFEGCPRERLQRNLDRAERKFDLMQELGAELMLLCSNVQADALGDRETLLGDLGLI
AERAGARGLRVGYEALAWGRHVKTWRDVWTLVQQVDHPALGVILDSFHTLALGDDPSGIV
EIPGERIFFVQLADAPVLAMDVLEWSRHFRCFPGQGEFDLAGFLAPVLQAGYSGPLSLEV
FNDGFRAAPPRGIAADGLRALRHLEEKTGQRLSASAPSAAVVPSLFTAPAAPRYEGIDFL
EFAVDEPHAVRLGSWLQQLGFAHVGQHRSKDVQLFRQGEINIVLNAEPYSFAHNYFEAHG
PSVCAMALKIDDEASALARACAFGGQPYRGLIGPNERQIPAVRAPDGGLIYLLESGAPGQ
SNYDIDFRLHAVTPAGAGLQRIDHMATALPAESLASWVLFYRSLFDFEADDELLLPDPYG
LMKCRAMRSPCGRVRLPLNTSQDRDTVIAQALSSYRGAGVHHVAFACDDIFAEVARAQAA
GVPLLQIPRNYYDDLAARFDFDDSLLAELARYNVLYDRDAEGGELFHVYTEPFEGRFFFE
ILQRCNGYAGYGTPNVAVRLAAMAKQCRRTAPAATALASAS