Protein Info for HP15_3935 in Marinobacter adhaerens HP15
Annotation: pyruvate carboxylase subunit B
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01960, pyruvate carboxylase subunit B [EC: 6.4.1.1] (inferred from 67% identity to alv:Alvin_1668)Predicted SEED Role
"Pyruvate carboxylase subunit B (biotin-containing) (EC 6.4.1.1)" (EC 6.4.1.1)
MetaCyc Pathways
- reductive TCA cycle I (7/11 steps found)
- incomplete reductive TCA cycle (4/7 steps found)
- gluconeogenesis III (7/12 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (10/18 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.4.1.1
Use Curated BLAST to search for 6.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PJY9 at UniProt or InterPro
Protein Sequence (599 amino acids)
>HP15_3935 pyruvate carboxylase subunit B (Marinobacter adhaerens HP15) MTKRKIHITDVILRDAHQSLIATRMRTEDMLPACEWLDQAGYWSLECWGGATFDACVRFL KEDPWERLRTLRKALPNTRLQMLLRGQNLLGYRHYGDDVVEAFVAKAAENGMDVFRIFDA LNDVRNLETSIKAVKKAGKHAQGTICYTVSPVHGTDAYLEQAKAMADMGADSIAIKDMAG LLTPAVTAELVGKLKQTLNLPVFLHSHATSGMAPMCQWAAMEAGVDHIDTALSAFAGGTS HPPTESLVAALHDAGLETGLNLELLDKATRHFREVRKKYHQFESAYNGVDTSVLLSQVPG GMMSNLANQLKEQGALDRIQDVFEEIPRVRKDLGYPPLVTPTSQIVGTQAVINVLAGKRY ESITNEVKKYLQGWYGKAPAAVDKSLQQRAVGKEDLIEERPANLIPRELDELRKQVGELA TSEEDVLTYAMFPDQAKAYLEQRRDGTLQPEPLEPIPTGSSGGSVSSKFKITVHGESYDI HVTGANPSGENERRFYMTVDGVPEEIHLASLGEDGEGARSSGGRATATKEGHVTTSMPGN IVDVLVKEGDEVQAGDPVLIIEAMKMETEVKATTAGKVSGVFIAKGDRVVPGEVLVEID