Protein Info for Psest_4064 in Pseudomonas stutzeri RCH2

Annotation: Lactate dehydrogenase and related dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 12 to 326 (315 residues), 99.3 bits, E=1.5e-32
• PF02826: 2-Hacid_dh_C
  • amino acids 110 to 297 (188 residues), 191 bits, E=1.2e-60

Best Hits

Swiss-Prot: 52% identical to DDH_HAEIN: 2-hydroxyacid dehydrogenase homolog (ddh) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03778, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 94% identity to psa:PST_0207)

MetaCyc: 51% identical to D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
D-lactate dehydrogenase. [EC: 1.1.1.28]

Predicted SEED Role

"D-lactate dehydrogenase (EC 1.1.1.28)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.28)

MetaCyc Pathways

• heterolactic fermentation (15/18 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• mixed acid fermentation (12/16 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• vancomycin resistance I (1/3 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (5/9 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (3/8 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.28

Sequence Analysis Tools

See L0GTB8 at UniProt or InterPro

Protein Sequence (329 amino acids)

>Psest_4064 Lactate dehydrogenase and related dehydrogenases (Pseudomonas stutzeri RCH2)
MRITLFSSKAYDRDSFLAANQAHGFDLHFLETRLDPDTAALAAGAEVVCPFVNDVLSAPV
LERLAAGGTRLIALRSAGYNHVDLPCAKRLGLPVVRVPAYSPHAVAEHTVALILALNRRL
PRAYNRIREGNFSLQGLTGFDLVGKRVGVVGSGQIGAVFARIMLGFGCEVQLYDPYPNPE
LERLGMRYVALDELLASSDIVSLHCPLTAQTHHLINQQSLATMKPRAMLINTGRGALIDT
PALIGALKSGQLGYLGLDVYEEEAGLFFEDHSGLPLQDDVLARLLSFPNVIVTAHQAFLT
EEALSAIAGTTLGNVAAWQAGQTSNQVSD