Protein Info for Psest_4062 in Pseudomonas stutzeri RCH2

Annotation: sulfate ABC transporter, permease protein CysW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 27 to 48 (22 residues), see Phobius details amino acids 70 to 96 (27 residues), see Phobius details amino acids 107 to 132 (26 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 208 to 233 (26 residues), see Phobius details amino acids 254 to 274 (21 residues), see Phobius details TIGR02140: sulfate ABC transporter, permease protein CysW" amino acids 25 to 282 (258 residues), 400.5 bits, see alignment E=3.3e-124 TIGR00969: sulfate ABC transporter, permease protein" amino acids 27 to 279 (253 residues), 316.7 bits, see alignment E=1.4e-98 PF00528: BPD_transp_1" amino acids 87 to 283 (197 residues), 55 bits, see alignment E=4.6e-19

Best Hits

Swiss-Prot: 50% identical to CYSW_ECOL6: Sulfate transport system permease protein CysW (cysW) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K02047, sulfate transport system permease protein (inferred from 91% identity to psa:PST_0209)

MetaCyc: 50% identical to sulfate/thiosulfate ABC transporter inner membrane subunit CysW (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate transport system permease protein CysW" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRW9 at UniProt or InterPro

Protein Sequence (291 amino acids)

>Psest_4062 sulfate ABC transporter, permease protein CysW (Pseudomonas stutzeri RCH2)
MTTASLSVTANSTANATRRGNALGRRLLIVAAWLVFALFLLLPLLIVVSEALKQGFGTFF
SAIFEPDAFAALKLTLLAVGISVPLNLVFGVAAAWCVSKYEFRGKSLLVTLIDLPFSVSP
VIAGLIYVLLFGAQGYFGEWLSDRDIQIIFAVPGIVLATLFVTVPFVARELIPLMQEQGS
TEEEAARLLGASGWQMFWHITLPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGLTNT
LPLHVEILYNEYNHVAAFSVASLLLGLALVILLLKQWSESRIARLHAKAEE