Protein Info for Psest_4061 in Pseudomonas stutzeri RCH2

Annotation: sulfate ABC transporter, permease protein CysT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 42 to 58 (17 residues), see Phobius details amino acids 64 to 84 (21 residues), see Phobius details amino acids 96 to 118 (23 residues), see Phobius details amino acids 134 to 154 (21 residues), see Phobius details amino acids 186 to 205 (20 residues), see Phobius details amino acids 240 to 264 (25 residues), see Phobius details TIGR00969: sulfate ABC transporter, permease protein" amino acids 4 to 267 (264 residues), 305.1 bits, see alignment E=4.6e-95 TIGR02139: sulfate ABC transporter, permease protein CysT" amino acids 7 to 270 (264 residues), 415.1 bits, see alignment E=1.3e-128 PF00528: BPD_transp_1" amino acids 74 to 269 (196 residues), 77.9 bits, see alignment E=4.4e-26

Best Hits

Swiss-Prot: 50% identical to CYST_SALTY: Sulfate transport system permease protein CysT (cysU) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02046, sulfate transport system permease protein (inferred from 96% identity to psa:PST_0210)

MetaCyc: 48% identical to sulfate/thiosulfate ABC transporter inner membrane subunit CysU (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate transport system permease protein CysT" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRB8 at UniProt or InterPro

Protein Sequence (271 amino acids)

>Psest_4061 sulfate ABC transporter, permease protein CysT (Pseudomonas stutzeri RCH2)
MSRSTSVIPGFGLTLGYTLVYLSLLVLIPLGALFLKTTELSWAQFIAIVSAPRVLAALKL
SFGTAFVAAVLNGIIGTLLAWVLVRYRFAGRKIIEAMIDLPFALPTAVAGIALTALYAPN
GPIGQFATDLGFKIAYTPLGITLALTFVTLPFVVRTIQPVLADIPREVEEAAACLGARPL
QVVRHVLLPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYDY
AGATAIGVLMLVVSFVLLLLINLLQRRIARS