Protein Info for Psest_4060 in Pseudomonas stutzeri RCH2

Annotation: sulfate/thiosulfate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 21 to 328 (308 residues), 459.3 bits, see alignment E=3.3e-142 PF13531: SBP_bac_11" amino acids 35 to 282 (248 residues), 142.4 bits, see alignment E=4e-45 PF01547: SBP_bac_1" amino acids 38 to 273 (236 residues), 68.5 bits, see alignment E=2.1e-22 PF13416: SBP_bac_8" amino acids 111 to 294 (184 residues), 41.6 bits, see alignment E=2.8e-14 PF13343: SBP_bac_6" amino acids 128 to 297 (170 residues), 32 bits, see alignment E=1.8e-11

Best Hits

Swiss-Prot: 64% identical to SUBI_SHIFL: Sulfate-binding protein (sbp) from Shigella flexneri

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 93% identity to psa:PST_0211)

MetaCyc: 64% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GT37 at UniProt or InterPro

Protein Sequence (332 amino acids)

>Psest_4060 sulfate/thiosulfate-binding protein (Pseudomonas stutzeri RCH2)
MSIRRFALAALASALIGSHALAADTLLNVSYDPTRELYVEFNAAFNKHWQAQGHEPVKVQ
QSHGGSGKQARAVIDGLRADVVTLALAGDIDELHRLGKLIPEDWQTRLPDASTPYTSTIV
FLVRKGNPKGIKDWDDLVKPGVEVITPNPKTSGGARWNFLAAWAYAQQKFGTEEQAQEFV
GTLYKNVPVLDTGARGSTVTFVNNGIGDVLLAWENEAFLALKEQGGENFEIIAPSLSILA
EPPVAVVDKNVERKGTREVAEAYLNYLYSEEGQRIAAKNFYRPRNASVAAEFKDQFPALK
LVTIDRDFGGWKTAQPKFFNDGGVFDQIYQTQ