Protein Info for HP15_3913 in Marinobacter adhaerens HP15

Annotation: multidrug resistance protein NorM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 transmembrane" amino acids 23 to 39 (17 residues), see Phobius details amino acids 66 to 87 (22 residues), see Phobius details amino acids 107 to 128 (22 residues), see Phobius details amino acids 143 to 161 (19 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 201 to 226 (26 residues), see Phobius details amino acids 251 to 278 (28 residues), see Phobius details amino acids 290 to 312 (23 residues), see Phobius details amino acids 332 to 351 (20 residues), see Phobius details amino acids 371 to 392 (22 residues), see Phobius details amino acids 402 to 423 (22 residues), see Phobius details amino acids 433 to 456 (24 residues), see Phobius details PF01554: MatE" amino acids 32 to 191 (160 residues), 102.8 bits, see alignment E=8e-34 amino acids 258 to 419 (162 residues), 114.8 bits, see alignment E=1.7e-37 TIGR00797: MATE efflux family protein" amino acids 32 to 436 (405 residues), 306.2 bits, see alignment E=1.6e-95

Best Hits

Swiss-Prot: 44% identical to NORM_SHEON: Probable multidrug resistance protein NorM (norM) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 86% identity to maq:Maqu_0260)

MetaCyc: 41% identical to multidrug efflux pump MdtK (Escherichia coli K-12 substr. MG1655)
RXN0-2561; TRANS-RXN-347; TRANS-RXN-348; TRANS-RXN-349

Predicted SEED Role

"Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PJW7 at UniProt or InterPro

Protein Sequence (473 amino acids)

>HP15_3913 multidrug resistance protein NorM (Marinobacter adhaerens HP15)
MDEIHQPLPEPRQPLVTRTLTEWRTLAILGGPILIAQLAQMANGVIDTIMAGHASAEDLA
AVGIGSSLWMPLFLFFMGMLGALQPIISGYNGAREVGKIMPATWQGLYLAAAGSVIMILL
LTHVHPVLALLNLEANTARITQGYLNAFAWGIPALLLMTALRGLTDGLGHTRVIMAFSVL
STLINLPMNYIFIYGKLGLPAMGGVGCGWATSISNGVAAAALLVYLNRSRVYRQFHLIAD
WVKPDLAGMRYILGIGVPIGFTIFVEASLFSVIALFLAPLGPVVVAGHQIALNVVSLLFM
LPLSIGMALTLRVSFLIGARAPATARLISRSSLILAAATALVFAILLFVFSREIAALYTS
DTEVQAVTVRLLMFAALFQIADVIQVTCISALRGYKDTRIPMFIMLFSFWGVGMPLGYVL
TFMDWLVPAMGAAGFWVGLTGGLASASILLGCRLFLFAPNSGQAVSAASSGVR