Protein Info for HP15_3880 in Marinobacter adhaerens HP15

Annotation: nitrite reductase (NAD(P)H), large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 835 PF07992: Pyr_redox_2" amino acids 5 to 286 (282 residues), 178.7 bits, see alignment E=6.3e-56 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 799 (794 residues), 1075.4 bits, see alignment E=0 PF13738: Pyr_redox_3" amino acids 70 to 266 (197 residues), 32.2 bits, see alignment E=2.5e-11 PF00070: Pyr_redox" amino acids 148 to 218 (71 residues), 61.7 bits, see alignment E=2.8e-20 PF18267: Rubredoxin_C" amino acids 319 to 384 (66 residues), 41.2 bits, see alignment E=4.7e-14 PF04324: Fer2_BFD" amino acids 422 to 469 (48 residues), 41.6 bits, see alignment 4.6e-14 PF03460: NIR_SIR_ferr" amino acids 558 to 620 (63 residues), 45 bits, see alignment 2.6e-15 PF01077: NIR_SIR" amino acids 630 to 768 (139 residues), 86.6 bits, see alignment E=4.6e-28

Best Hits

Swiss-Prot: 63% identical to NIRB_KLEOX: Nitrite reductase [NAD(P)H] large subunit (nasB) from Klebsiella oxytoca

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 86% identity to maq:Maqu_0172)

MetaCyc: 58% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PJT4 at UniProt or InterPro

Protein Sequence (835 amino acids)

>HP15_3880 nitrite reductase (NAD(P)H), large subunit (Marinobacter adhaerens HP15)
MSKKTLIVIGNGMVGHHFLEQFCASPAAADFEIIVFGEEKQLAYDRVHLSEYFGGSTHAD
LAMGTLEWYTEQGIQLHLNEQVTGINREDRTLATPRGSYRYDELVLATGSYPFVPPIEGN
DRPHCLVYRTLDDLDAIRASSEGARAGVVVGGGLLGLEAANALKSLGLEAHVVEFAPRLM
PVQLDADGGALLRHKIEELGVQVHTEKATTAITEGEDARLRMNFSDESFLETDLIVFSAG
IRPQDALARASGLAIGERGGIVINNHCTTSDPHVHAIGECALWEQKIFGLVAPGYTMART
LSAALNGDRETAFTGADMSTKLKLLGVDVGSIGDAHAQTPGARNIRFNDEQAGHYRRMVI
SEDGKTLLGAILVGDNSHYDTLLQYALNGITLPENPETLILPESQGGAPALGPDALPETA
SICSCHNVTKGDICGAINAGCSDLGSVKAETKASTGCGGCTALLKNVVDSELEKRGVEVS
KDLCEHFPYSRQELFHLVKVNGIRTFGTLISQHGRGHGCDICKPAVASILATCWNEHILA
TDHVPLQDTNDTFMANMQKNGTYSIVPRIPGGEITPDKLIVLGEVAKEYDLYTKITGGQR
VDLFGATLSQLPEIWEKLIAAGFETGHAYGKSLRTVKSCVGNTWCRYGVQDSVGMAIRLE
DRYKGLRAPHKVKMAVSGCTRECAEAQSKDFGVIATENGWNLYVCGNGGMRPRHADLFAT
DLSDEELIRTIDRVVMFYVRTADRLQRTSVWMENLEGGLEYLKQVVLEDSLGIGAELEEH
MEGIVGTYQCEWKTAVEDPEKRKRFREFVNAPSQSDPVQEWTVERGQRRPVLESA