Protein Info for Psest_3998 in Pseudomonas stutzeri RCH2

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 6 to 519 (514 residues), 736.2 bits, see alignment E=9.3e-226 PF04262: Glu_cys_ligase" amino acids 10 to 382 (373 residues), 560.6 bits, see alignment E=6.1e-173

Best Hits

Swiss-Prot: 89% identical to GSH1_PSEU5: Glutamate--cysteine ligase (gshA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 89% identity to psa:PST_0276)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRR8 at UniProt or InterPro

Protein Sequence (525 amino acids)

>Psest_3998 glutamate--cysteine ligase (Pseudomonas stutzeri RCH2)
MSALLSHRLALLAESTHLPLLSQCLHGIERECLRVDARGQLAMTPHPAALGSALTHPQIT
TDYSEALLEFITGTDQDPQNTLAELEAIHRFSYAKLGDEFLWSPSMPCPLPSEADIPIAE
YGSSNIGRLKHVYRQGLALRYGKTMQCIAGIHYNFSLPEALWPVLQADDGDASSAQDYRS
TRYIAVIRNFRRYSWLLMYLFGASPALDAGFLRGRQHQLETLGADTLYLPYATSLRMSDL
GYQNNAQAGLTPCYDNLDSYTESLFRAVSTPYAPYETMGIKDAAGNWQQLNTNVLQIENE
YYSNIRPKRVTATGERPLQALRARGIQYIEVRCLDINPFLPLGIDASEAHFLDAFLLFCA
LNDSPCLADGECSVATENFLKVVKDGRRPGLELQRCGENVQLSDWAEQLLDEIAQVAALL
DRSHGDARHATALAEQRAKVADSSLTPSARVLDELRRSGESFSQFSMRQTQAHADYFRSR
APSPAELAQFEAAAQQSLERQAAMEAGDELDFDSFVAEYQRSLSL