Protein Info for PGA1_65p00290 in Phaeobacter inhibens DSM 17395

Annotation: algA: alginate biosynthesis protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 473 TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 2 to 471 (470 residues), 579.1 bits, see alignment E=3.6e-178 PF00483: NTP_transferase" amino acids 4 to 282 (279 residues), 158.2 bits, see alignment E=4.4e-50 PF01050: MannoseP_isomer" amino acids 318 to 468 (151 residues), 201.9 bits, see alignment E=7.9e-64 PF07883: Cupin_2" amino acids 385 to 452 (68 residues), 50.4 bits, see alignment E=2.2e-17

Best Hits

Swiss-Prot: 46% identical to MANC_SALTY: Mannose-1-phosphate guanylyltransferase ManC (manC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] (inferred from 83% identity to sit:TM1040_3778)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DX61 at UniProt or InterPro

Protein Sequence (473 amino acids)

>PGA1_65p00290 algA: alginate biosynthesis protein (Phaeobacter inhibens DSM 17395)
MITPILLCGGSGTRLWPLSRKSYPKQFVPLVGETTLFQASALRLSGEGFAAPMVLTNSDF
RFIVTEQLAEAGIDPGAILIEPAGRNTAPAVLAAALWLRARDPEGLMLVAPSDHVVPDAA
AFRAAVAAAEPAARAGELVTFGIKPDRAETGYGYLELDGDPGDFSPKVIGLKRFVEKPDL
ATAEDMLASGQFLWNAGIFLFSVKAIIAAFETHAPDLMVPVQGAVDQGEPDLGFLRLDPA
AWDGAADISIDYAVMERAENLAVVPYAGGWSDLGGWDAVWRESGPDGDGIVTQGRATAIE
CSNSLLRSEDDGLEVVGIGLKDVIAVAMPDAVLVADASRAQEVKKAVAALKAKSARQAEA
FPKDHRPWGWFESLVVGERFQVKRIHVHPGAALSLQSHHHRSEHWIVVEGTAKVTVDDSV
QLVSENQSVYIPLGAVHRMENPGKVPMVLIEVQTGSYLGEDDIIRYEDIYARK