Protein Info for Psest_3962 in Pseudomonas stutzeri RCH2

Annotation: haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 PF00702: Hydrolase" amino acids 10 to 182 (173 residues), 73.3 bits, see alignment E=5.5e-24 PF12710: HAD" amino acids 13 to 142 (130 residues), 39.3 bits, see alignment E=1.5e-13 PF13419: HAD_2" amino acids 13 to 188 (176 residues), 48.3 bits, see alignment E=2e-16 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 83 to 188 (106 residues), 47.7 bits, see alignment E=1.9e-16

Best Hits

KEGG orthology group: K07025, putative hydrolase of the HAD superfamily (inferred from 88% identity to psa:PST_0317)

Predicted SEED Role

"FIG001957: putative hydrolase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR09 at UniProt or InterPro

Protein Sequence (226 amino acids)

>Psest_3962 haloacid dehalogenase superfamily, subfamily IA, variant 3 with third motif having DD or ED (Pseudomonas stutzeri RCH2)
MTANLPWSDIDTVLLDMDGTLLDLHFDNHFWLELLPQRYAELHGISRAMAELELAPLFNE
HVGKLTWYCLDYWTRELNLPIREMKREIAELIALRPSADDFLAALREAGKRVVLITNAHR
DSLSLKLEKVELAPWFDRLISSHDYGYPKEEPQFWHALRQDLDFDPARALFIDDSLPILR
SAGRFGIAHLLAVREPDSRRPAKDTEEFAAVGDYRELVRNLLGEQA