Protein Info for Psest_3957 in Pseudomonas stutzeri RCH2
Annotation: glycogen/starch/alpha-glucan phosphorylases
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 96% identity to psa:PST_0323)Predicted SEED Role
"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)
MetaCyc Pathways
- glycogen degradation I (7/8 steps found)
- sucrose biosynthesis II (6/8 steps found)
- starch degradation V (3/4 steps found)
- glycogen degradation II (4/6 steps found)
- starch degradation III (2/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.1
Use Curated BLAST to search for 2.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GR06 at UniProt or InterPro
Protein Sequence (816 amino acids)
>Psest_3957 glycogen/starch/alpha-glucan phosphorylases (Pseudomonas stutzeri RCH2) MTQETAIATAEEVAAFRERVLRKLTYSVGKDPENASDYDWFHAVALATRDSTIDRWMDCT REAYNSGQKRVYYLSLEFLIGRLLVDSLSNLGLFEVAREALAGLDVDIDRIRLLEPDAAL GNGGLGRLAACFMESMATLGVVSHGYGIRYDHGLFRQAIVDGWQHEQTETWLDFGNPWEF ERSEVKYLIGFGGSVTATTNDKGEVQHFWHWAEGVRAIAYDTPIVGWRGAGVNTLRLWRA RPEADFHLARFNAGDHIGAAAEEARAESISRVLYPADSTEAGQELRLRQEYFFVAASLQD LLRRHIKQRGSLDSLPEYTAIQLNDTHPAIAVAELMRQLVDVHSYEWQQAWRLTTATLSY TNHTLLPEALETWPVGLMERLLPRHMQIIYLINAHHLDCLRERGIHDAELLRSVSLIEED HGRRVRMGNLAFLGSHSTNGVSALHTQLMRKTVFTDLHGLYPQRINSKTNGITFRRWLYQ ANPQLTQLLVEHLGEEVLDQPETRLRELEPFAEQAAFRRRFAEQRLANKRHLANVIQERL GISVDPNALFDVHVKRIHEYKRQLLNLLHTVALYQAIRSDPGGNWVPRVKIFAGKAAASY HQAKLIIKLTNDIANTINADPTVRGLLKVVFLPNYNVSLAEDIIPAADLSEQISTAGLEA SGTSNMKFALNGALTIGTLDGANVEMSEQIGLEHMFIFGLTAQQVNARKQANEYNAEAII GASPRLSEVLSAIRGGAFSPGDAGRYSGLVDGISWHDTFMVCADFEAYWQAQLDVEARWR DPERWWRSSVLNTARTGWFSSDRTIREYAREIWKVM