Protein Info for Psest_3943 in Pseudomonas stutzeri RCH2

Annotation: Phosphoribosyl-AMP cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 94% identical to HIS3_PSEU5: Phosphoribosyl-AMP cyclohydrolase (hisI) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01496, phosphoribosyl-AMP cyclohydrolase [EC: 3.5.4.19] (inferred from 94% identity to psa:PST_0337)

Predicted SEED Role

"Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19)" in subsystem Histidine Biosynthesis (EC 3.5.4.19)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRJ3 at UniProt or InterPro

Protein Sequence (131 amino acids)

>Psest_3943 Phosphoribosyl-AMP cyclohydrolase (Pseudomonas stutzeri RCH2)
MNWLDEINWNADGMVPAIAQDYQSGRVLMMAWMNREALALTAQEGRAIYWSRSRGKLWRK
GEESGHVQRLHELRLDCDADVIILMVEQIGGIACHTGRESCFYRVFENGSWKVVEPVLKD
PHAIYAEHKHE