Protein Info for Psest_3927 in Pseudomonas stutzeri RCH2

Annotation: thiazole biosynthesis/tRNA modification protein ThiI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 371 (368 residues), 331.6 bits, see alignment E=6.3e-103 PF02926: THUMP" amino acids 28 to 165 (138 residues), 64.7 bits, see alignment E=2.1e-21 PF02568: ThiI" amino acids 179 to 370 (192 residues), 211.6 bits, see alignment E=1.8e-66 TIGR04271: thiazole biosynthesis domain" amino acids 385 to 483 (99 residues), 121.6 bits, see alignment E=1.2e-39 PF00581: Rhodanese" amino acids 411 to 481 (71 residues), 22.8 bits, see alignment E=2.1e-08

Best Hits

Swiss-Prot: 84% identical to THII_AZOVD: tRNA sulfurtransferase (thiI) from Azotobacter vinelandii (strain DJ / ATCC BAA-1303)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 95% identity to psa:PST_0354)

MetaCyc: 51% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"tRNA S(4)U 4-thiouridine synthase (former ThiI) / Rhodanese-like domain required for thiamine synthesis" in subsystem Thiamin biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GQX2 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Psest_3927 thiazole biosynthesis/tRNA modification protein ThiI (Pseudomonas stutzeri RCH2)
MKLIVKVFPEITIKSPPVRKGFIRQLAKNIRTVLRELDPDLRVEGVWDNIEVETALSEPK
LLREMIERLRCTPGIAHCLEVHEYPLGDLDDIVARCKAHYAERLPGKIFAVRCKRAGKHP
FSSMDVERHVGSQLRQQCAAAGISLKKPEVEVRMEIRDQRLFVVHEQHDGIGGYPLGALE
QTLVLMSGGFDSTVAAYQMMRRGLMTHFCFFNLGGRAHELGVMEVAHYLWKKYGSSHRVL
FISVPFEEVVGEILQKVDNSQMGVVLKRMMLRVSTQIAERLHIDALVTGEAISQVSSQTL
PNLSVIDSATDMLVLRPLIASHKQDIIDTAFEIGTAEFAKNMPEYCGVISVNPTTKAKRY
RIEHEEKQFDMAILERALANARQVAIDRVIDELGQDLQVEEVVEPSAGQVIIDIRHPDAA
EDEPLELAGIEVQTLPFYALNNRFKELDENRQYLLYCDKGVMSRLHAHHLLSEGHVNVRV
YRPA