Protein Info for Psest_3924 in Pseudomonas stutzeri RCH2

Annotation: Na+/citrate symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 25 to 44 (20 residues), see Phobius details amino acids 50 to 69 (20 residues), see Phobius details amino acids 79 to 100 (22 residues), see Phobius details amino acids 117 to 137 (21 residues), see Phobius details amino acids 145 to 169 (25 residues), see Phobius details amino acids 175 to 194 (20 residues), see Phobius details amino acids 206 to 231 (26 residues), see Phobius details amino acids 260 to 279 (20 residues), see Phobius details amino acids 289 to 307 (19 residues), see Phobius details amino acids 322 to 341 (20 residues), see Phobius details amino acids 350 to 371 (22 residues), see Phobius details amino acids 382 to 403 (22 residues), see Phobius details amino acids 415 to 436 (22 residues), see Phobius details PF03390: 2HCT" amino acids 24 to 429 (406 residues), 511.3 bits, see alignment E=8.7e-158

Best Hits

Swiss-Prot: 38% identical to CITN_SALTY: Citrate-sodium symporter (citP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 96% identity to psa:PST_0357)

MetaCyc: 40% identical to citrate:Na+ symporter (Vibrio cholerae O1 biovar El Tor str. N16961)
TRANS-RXN-501

Predicted SEED Role

"Malate Na(+) symporter" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNQ9 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Psest_3924 Na+/citrate symporter (Pseudomonas stutzeri RCH2)
MNRTTLSPAVPEGTPNVSLLSQRIFNLPLPLFAIALLVMAAAIVTDTLPTGMIGALLVMM
LLGELLGFAGDRLPIIRTYLGGGAIMALFGAASMVYFGWLPAAVADDVASFMKGGGFLDF
YIAALITGSILGMDAKVLVKVGSRYALPLLCSVLFAALFAMAVGALLGFSPQDAVVVIAM
PIMGGGMGAGAVPMSQIYEQLLGQPASYYISILVPALALGNVFAIIIAGLLNGLGNRYPS
LTGNGQMMPGVDVSDKEGPITLPALGIGLVAALSFFIAGQILGKFVPLHPYALMIVLVAL
LKVSNLVPESINDAASQWFRFVARNWTFALLFGIGVAFTDLGQVLDAISLTYVLIVFAVV
AGAAFGAGLVGRLVGFYPIESAITAGLCMANMGGTGDVAVLSAARRMSLMPFAQISSRLG
GALILLISSVVVPLFFT