Protein Info for Psest_3923 in Pseudomonas stutzeri RCH2

Annotation: Signal transduction histidine kinase regulating citrate/malate metabolism

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 534 transmembrane" amino acids 7 to 28 (22 residues), see Phobius details amino acids 149 to 167 (19 residues), see Phobius details amino acids 174 to 193 (20 residues), see Phobius details PF17203: sCache_3_2" amino acids 31 to 171 (141 residues), 87.3 bits, see alignment E=3.2e-28 PF00989: PAS" amino acids 213 to 313 (101 residues), 36.5 bits, see alignment E=1.3e-12 PF08448: PAS_4" amino acids 220 to 316 (97 residues), 24.7 bits, see alignment E=6.8e-09 PF02518: HATPase_c" amino acids 422 to 524 (103 residues), 61 bits, see alignment E=4.2e-20 PF14501: HATPase_c_5" amino acids 424 to 512 (89 residues), 43.4 bits, see alignment E=8.1e-15

Best Hits

Swiss-Prot: 40% identical to MALK_BACSU: Sensor histidine kinase MalK (malK) from Bacillus subtilis (strain 168)

KEGG orthology group: K02476, two-component system, CitB family, sensor kinase [EC: 2.7.13.3] (inferred from 88% identity to psa:PST_0358)

Predicted SEED Role

"Two-component sensor histidine kinase, malate (EC 2.7.3.-)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRH5 at UniProt or InterPro

Protein Sequence (534 amino acids)

>Psest_3923 Signal transduction histidine kinase regulating citrate/malate metabolism (Pseudomonas stutzeri RCH2)
MRLRLNTLISLLVAVMVVLALALALWLFNVQLQDTLEEGQASRVTNLAQSVAARDDVQLA
LAGPADFNADNSLQREIDGLRQRLGVDFIVVMNPRALRLTHPQPTLIGQAFRGDDEGPVL
AGETYASRADGSLGRSIRGFAPVLNDEGAVLGAVSVGVTLASLGELLKAHQRGVVLGVLA
LMLVTALGAHMLARYIKRVLLGLEPYEITWLVEERQAMLASVREGVLAVDDQARITLVNP
AAERLLASTGLGKPPLGRPIAEYLPNSGLPEVLASATEQRDREFSLNGRAILANRAPIRH
QGRVIGAIATFRDKSEVNALAEQLTGVSRYAEALRAATHEFKNKLHVLLGLAQMGDLDAL
RAYLRDLADHQLAPATSLVEGIGEPVLAGFLLGKQSEARERGIDFQVDVEHPLPAAAPEQ
IHGLVTILGNLLENAFEAVAEQDERRVNLTLNYDEALLSLHVQDSGGGIEATVRGQIFER
GVSTKGERRGIGLAAVQEQVEAWGGSLAVYSEAGRGSLFEVELSYRTAAEGEAP