Protein Info for PGA1_262p02280 in Phaeobacter inhibens DSM 17395

Annotation: Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 152 to 238 (87 residues), 41.6 bits, 2.8e-14
  • amino acids 270 to 336 (67 residues), 35.2 bits, 2.9e-12
• PF02096: 60KD_IMP
  • amino acids 407 to 581 (175 residues), 73.5 bits, E=4e-24
• PF01326: PPDK_N
  • amino acids 672 to 781 (110 residues), 81.7 bits, E=1.2e-26
  • amino acids 806 to 851 (46 residues), 27.2 bits, see alignment (E = 4.6e-10)
• PF00391: PEP-utilizers
  • amino acids 1257 to 1322 (66 residues), 49.2 bits, 6.3e-17

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• gluconeogenesis I (12/13 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See I7E6M3 at UniProt or InterPro

Protein Sequence (1341 amino acids)

>PGA1_262p02280 Phosphoenolpyruvate synthase/pyruvate phosphate dikinase (Phaeobacter inhibens DSM 17395)
MIPKCIRFHTPVSRLSLAVLFSSALAPAAAWAIPSPELVIGSVSSLSQVLAVGVAMVSGL
GAVVAAKLGLQPKGKSEAKRYPIKLITGLVLAALALAMLNYWQFTTQRAAEQARLQATLV
RPAQFDGTKIKDAALKETSFSRQSDHPLAMSTADAAAALDDGQTLFYDIRETGENAMGTL
PGATHIRFPDFLQSRPVQPGQKVVLFCHNGNRSSETCAKLAAMGIDCSFIAGGIEKWIVE
GRDFSDTDVKTLSDLRAIPEYPGRDVLLSTADFTDLMNTEDLQIVDTRYPGDFDAGHLPG
AVNIPIRKMTTADLMQRISELDPSKPTLAACYDRRSCFMSQVLGLELAQKGFDFRGRYTL
PWEYFIAPAPKPHVQAWLADQQSGLWDKAISALAAALLWVHERSHILLGLLALSLITRIM
ILPVALKSERDQITTNDTADEMKALKERLADDPVRKARAVQAFYADKGLTPMRNLTALLF
LPVMMLGVSAAQEASASLQTPFLWMADLGAADPLFILPVLFCALAAVYLLWAVAKTPRQK
TLWMVLGMPALFAMVFTLTAAANAYLCFSLTLLLVQRAYVTGLHRKLGEAFALRAHKHRL
AKLPRGVIPMGYTEELEHAGNKALRLSILRNAGLPVPGGVIVRSDAIHDYREMSDAQKNA
FAAKVFALAGGKPVAVRSSASNEDGADQSFAGVFESVLDVTADTMRAALDDVVESFSSDR
AASYAGDGDSADQGNIVVQEMVQADYAGVMFTQDPMAPGMCMIEWVEGCGEDLVSGRVTP
ISLRFGRYTGLPASEDQDQTLDMAPLLALGQQIEETFGAPQDVEWAYANGEFQIVQSRDI
TTLSLGTEQEQARLAEWRDLLDHYGNADPDQTILEQDEMSEVLPRPTPLSFSLMGSLWSP
GGSVDLACRALSVPYNLPEGRAGHLVNLFGKTYVDVDLKEQMTLKLTATKAKQLRKQARP
MIDHFHAEVLPRLSDQLAFWQAVDYAALPLPKQLEAIATLRDLFVTDIYVEAEKVNIIAG
FTMGEASAAAAGDPALRAHLMHADLPNAPSSLLASCTGPMAESRAQVLMGHRSIFDYELS
APRYKEAPSLLISLLDSSVEPISGTVTPPADLPEDLRDTLDLAIAYQDLKEQAKHEALRV
LAELRRALLALGSRSGLDDLVFYLAMDDIMTGNWDQPEALKDRATARKHREELRKPSAPT
SVNLTLRDCELLSLGAQAGSGDGSLGGTCVAGSGRVEGRVFWVEDETAIGPDVFDGFQES
DILACRMINPAWLPYVQRSGAVLSEVGGWLSHMAIVAREKDILMLVACKGLDQLAHGELI
TVAEDGSITQSNASDLKAASA