Protein Info for Psest_0376 in Pseudomonas stutzeri RCH2

Annotation: glucarate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 TIGR03247: glucarate dehydratase" amino acids 7 to 446 (440 residues), 867 bits, see alignment E=1.2e-265 PF02746: MR_MLE_N" amino acids 44 to 138 (95 residues), 23.4 bits, see alignment E=5.8e-09 PF13378: MR_MLE_C" amino acids 189 to 406 (218 residues), 167.6 bits, see alignment E=3.6e-53

Best Hits

Swiss-Prot: 80% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida

KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 86% identity to pmk:MDS_1253)

MetaCyc: 80% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]

Predicted SEED Role

"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.40

Use Curated BLAST to search for 4.2.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGW1 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Psest_0376 glucarate dehydratase (Pseudomonas stutzeri RCH2)
MTIAATGTPRITELTVVPVAGQDSMLMNLSGAHGPWFTRNILILKDSAGHVGVGEVPGGE
AIRQTLEDARVLLVGEPIGQYNALLNRARRAFADRDSGGRGLQTFDLRIAIHAVTALESA
LLDLLGQHLEVPVAALLGDGQQRDEVEMLGYLFFIGDRNKTDFGYRDESGADDAWFRLRN
EEAMTPESIVRQAEAAYERYGFKDFKLKGGVLRGEEEVEAIRALAARFPDARVTLDPNGA
WSLDEAIGLCRDLHGVLAYAEDPCGAENGYSGREVMAEFRRATGLPTATNMIATDWRQMS
HTVQLNSVDIPLADPHFWTMAGSVRVAQMCADFGLTWGSHSNNHFDISLAMFTHVAAAAP
GRVTAIDTHWIWQDGQHLTLEPLKIVGGKVAVPQKPGLGVELDWDALQQAHAHYQEKGLG
ARDDAIAMQYLIPDWTFNNKKPCMVR