Protein Info for PGA1_262p01500 in Phaeobacter inhibens DSM 17395

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 988 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 12 to 987 (976 residues), 1335.1 bits, see alignment E=0 PF13510: Fer2_4" amino acids 20 to 104 (85 residues), 91.1 bits, see alignment E=1.5e-29 PF07992: Pyr_redox_2" amino acids 174 to 353 (180 residues), 30.7 bits, see alignment E=9.1e-11 PF00890: FAD_binding_2" amino acids 174 to 210 (37 residues), 23.6 bits, see alignment (E = 1.1e-08) PF12831: FAD_oxidored" amino acids 174 to 214 (41 residues), 34.3 bits, see alignment (E = 6.8e-12) PF13450: NAD_binding_8" amino acids 177 to 215 (39 residues), 23.3 bits, see alignment (E = 2.5e-08) PF17806: SO_alpha_A3" amino acids 503 to 587 (85 residues), 114.1 bits, see alignment E=1.2e-36 PF01571: GCV_T" amino acids 602 to 874 (273 residues), 287.5 bits, see alignment E=3.1e-89 PF08669: GCV_T_C" amino acids 895 to 980 (86 residues), 40.3 bits, see alignment E=9.7e-14

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 83% identity to sit:TM1040_3377)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DWL8 at UniProt or InterPro

Protein Sequence (988 amino acids)

>PGA1_262p01500 sarcosine oxidase subunit alpha (Phaeobacter inhibens DSM 17395)
MSRRSVLKNRLSGGLIDHDAPLSFQFDGKTYEGHQGDTLASALLANGVQLMGRSFKYHRP
RGVLTAGSEEPNALVELRSGGRQEPNTRATVVELFAGLEARSQNRWPSLDHDMMAINDRF
SNFLTAGFYYKTFMWPKAFWEKLYEPIIRKAAGLGSLTGEADPDAYDKGYLHCDLLIIGA
GPTGLAAALTAGRAGAQVILAEEDYRLGGRLLSETKALSEMSGTDWVAQTQAELAALPNV
RVMPRTTVFGAYDHGIYGAVERNADHVPTPTAGKPRQTLWRIYSKRALIATGAIERPIAF
ENNDRPGVMLAGAARAYANRWAATPGQSVVIFANGDDAYHTARDLMAKGVEVPAIVDVRD
GAPTLPGTEVLAGAEIIDTKGRLGLSSVTVRLANGQTRTIACDALAVSGGWNPNLGLTCH
QRGRPVWSDAIQSFVPGTGLPAGQLVAGAAMGGMTTAQALKGGAERAVEALGDLGISARA
ADLPQAEDAPVSLTPFWHVKGAKRAWLDFQNDVTVKDVKLAHQENFTSVEHLKRYTTLGM
ATDQGKTSNLGALAAMAELTGKSIPDTGTTIFRPPYTPVAMGALAGRAVGKDFHPTRLTP
SHKWAEEQGAVFVEVGNWLRAQWFPREGETHWRQSVDREVLQTRNSVGICDVTTLGKIDV
QGADAATFLNMVYANGFAKLPVGKVRYGLMLREDGVAYDDGTAARLAEDHFVVTTTTANA
VLVYRNMEFVRQCLCPDLDVQLISTTEAWAQYAVAGPNARKLLQKIVDPEFDISNEAFPF
MGCGEVTVCGGCRARLFRISFSGELAYEIAVPTRYGDALMREMMAAGEEFDVTPYGTEAL
GVMRIEKGHAAGNELNGTTSALNLGLGRMVSTKKDCIGNVLSRRDGMNKENALNLVGVRP
VKSSDGVPAGGHLMATDGPVDAAHDQGYVTSAAYSPILQSAIGLAFVKGGANRMGERLRL
VNPLQNQTVLVEIVSPHFVDPEGEKLRA