Protein Info for Psest_3810 in Pseudomonas stutzeri RCH2

Annotation: orotate phosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 96% identical to PYRE_PSEU5: Orotate phosphoribosyltransferase (pyrE) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00762, orotate phosphoribosyltransferase [EC: 2.4.2.10] (inferred from 96% identity to psa:PST_0466)

MetaCyc: 66% identical to orotate phosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Orotate phosphoribosyltransferase. [EC: 2.4.2.10]

Predicted SEED Role

"Orotate phosphoribosyltransferase (EC 2.4.2.10)" in subsystem De Novo Pyrimidine Synthesis (EC 2.4.2.10)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
UMP biosynthesis I (6/6 steps found)
UMP biosynthesis II (6/6 steps found)
UMP biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GSC1 at UniProt or InterPro

Protein Sequence (213 amino acids)

>Psest_3810 orotate phosphoribosyltransferase (Pseudomonas stutzeri RCH2)
MQAYQREFIRFAIERGVLRFGEFTLKSGRTSPYFFNAGLFNSGGALAQLGRFYASAVMQS
GLDFDVIFGPAYKGIPLGAATAIALAEQHQRDLPWCFNRKEAKDHGEGGTLVGAPLAGRV
LIVDDVITAGTAIREVMQIIQAQNAEAAGVLIALNRQEKGQGELSAIQEVERDYRMPVIS
IVSLEQVLEYLADDVQLKQYLPAVQAYREQYGI