Protein Info for HP15_3677 in Marinobacter adhaerens HP15

Annotation: bifunctional protein GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 1 to 421 (421 residues), 576.1 bits, E=2.6e-177
• PF00483: NTP_transferase
  • amino acids 1 to 127 (127 residues), 36.5 bits, E=8e-13
• PF12804: NTP_transf_3
  • amino acids 1 to 91 (91 residues), 41.3 bits, E=3.8e-14
• PF00132: Hexapep
  • amino acids 235 to 266 (32 residues), 30.6 bits, see alignment (E = 3.9e-11)
  • amino acids 362 to 396 (35 residues), 32.5 bits, 1e-11
• PF14602: Hexapep_2
  • amino acids 362 to 396 (35 residues), 28.4 bits, 2.2e-10

Best Hits

Swiss-Prot: 70% identical to GLMU_MARHV: Bifunctional protein GlmU (glmU) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 70% identity to maq:Maqu_3873)

MetaCyc: 53% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• chitin biosynthesis (5/9 steps found)
• peptidoglycan recycling I (8/14 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See E4PHE0 at UniProt or InterPro

Protein Sequence (422 amino acids)

>HP15_3677 bifunctional protein GlmU (Marinobacter adhaerens HP15)
MLQHVINTAHQLRAEKIHTVIGHGAYQVQELIEDDGIHWVEQTERLGTGHAVAQVLDYLP
DDARVLVLYGDVPLIQEPTLATLVSHVDADSLGLLTVTMDDPTGYGRIVRDDRGRVTSIV
EQKDASTEQKGIREVNTGIMGVMASHLKAWVPQLSNDNAQGEYYLTDIISLAAGDEVDVV
VNQPQDLFEVQGVNNRVQLAELERWFQRHQAERLMLAGATLADPERVDVRGSLQVGTDVF
IDINVVFEGEVKIGNNVRIGPGCIVKDTEVGDGTEIKAYSVIESSKIGENGQIGPYARFR
PGNYLGANTKVGNFVELKKATVGEGSKINHLSYVGDATLGARVNVGAGTITCNYDGANKY
QTVIGDGVFVGSNCSLVAPVTVAAEATIGAGSTITRDVADHELAVARGRQRNIAGWEKPK
KH